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Algae and cyanobacteria are genetically engineered to have lower RUBISCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) content in order to grow more efficiently at elevated carbon dioxide levels while recycling industrial CO2 emissions back to products, and so as not to be able to grow outside of cultivation.

Titel:	Decreasing RUBISCO content of algae and cyanobacteria cultivated in high carbon dioxide
Autor/in / Beteiligte Person:	Schatz, Daniella ; Gressel, Jonathan ; Eisenstadt, Doron ; Ufaz, Shai
Link:	Zum Volltext
Veröffentlichung:	2010
Medientyp:	Patent
Sonstiges:	Nachgewiesen in: USPTO Patent Applications Sprachen: English Document Number: 20100081177 Publication Date: April 1, 2010 Appl. No: 12/584571 Application Filed: September 08, 2009 Assignees: TransAlgae Ltd (Rehovot, IL) Claim: 1. A method to over produce homologous or heterologous compounds in cyanobacteria or algae cells, said method comprising the steps of: a) Reducing amount of RUBISCO protein in the cells by genetically transforming the cells; b) Transforming the cells with a gene of interest under control of constitutive or inducible promoter to express production of the compound; and c) Cultivating the transgenic cell line in elevated carbon dioxide concentrations. Claim: 2. The method of claim 1, wherein reducing the amount of RUBISCO is achieved by transforming the cells with a transformation vector comprising rbcS or rbcL encoding polynucleotides in an antisense or in an RNAi-construct under a constitutive promoter. Claim: 3. The method of claim 2, wherein the transformation vector also comprises a polynucleotide sequence encoding the gene of interest. Claim: 4. The method of claim 1, wherein the cyanobacteria is selected from the group consisting Synechococcus PCC7002, Synechococcus WH-7803, and Thermosynechococcus elongatus BP-1. Claim: 5. The method of claim 4, wherein the cells are transformed by electroporation, microporation or by particle bombardment. Claim: 6. (canceled) Claim: 7. (canceled) Claim: 8. (canceled) Claim: 9. The method of claim 1, wherein the gene of interest encodes proteins. Claim: 10. The method of claim 9, wherein the proteins are pharmaceutical proteins or industrial proteins. Claim: 11. The method of claim 10, wherein the protein is human insulin. Claim: 12. The method of claim 10, wherein the protein is thermostable phytase. Claim: 13. The method of claim 9, wherein the proteins enhance algal or cyanobacterial growth. Claim: 14. The method of claim 13, wherein the proteins are rate limiting enzymes of photosynthesis. Claim: 15. The method of claim 14, wherein the enzyme is selected from the group consisting of fructose-1,6,-bisphosphate aldolase, choloplast triosephosphate isomerase and acetyl CoA carboxylase. Claim: 16. The method of claim 9, wherein the proteins are storage proteins. Claim: 17. The method of claim 16, wherein the protein is 15 Kd zein or BHL8. Claim: 18. The method of claim 1, wherein the gene of interest is encoding of production of oils or lipids. Claim: 19. The method of claim 18, wherein the gene of interest encodes acetylCo-carboxylase. Claim: 20. A method to cultivate algae and cyanobacteria under high CO2 concentration, wherein the algae and cyanobacteria are genetically modified to express low amounts of RUBISCO protein. Claim: 21. A method to prevent establishment of transgenic algal or cyanobacterial cells in the natural environment by down regulating the amount of RUBISCO protein in the cells by genetic modification, whereby the cells become incapable to grow in ambient carbon dioxide concentrations. Claim: 22. A transgenic alga or cyanobacterium, transformed with a vector comprising rbcS or rbcL encoding polynucleotides in an antisense or an RNAi-construct under a constitutive promoter; whereby the transgenic alga or cyanobacterium expresses reduced content of RUBISCO protein. Claim: 23. The transgenic alga or cyanobacterium of claim 22, wherein the alga or cyanobacterium also transformed to express a gene of interest. Claim: 24. The transgenic alga or cyanobacterium of claim 23, wherein the gene of interest and the antisense or RNAi construct are transformed in tandem. Claim: 25. The method of claim 1, wherein the alga is selected from the group consisting of Chlamydomon reinhardtii, Pavlova lutheri, Isochrysis CS-177, Nanochloropsis CS-179, Nanochloropsis CS-24 Nanochloropsis salina CS-190, Tetraselmis suecica, Tetraselmis chuii and Nannochloris sp Claim: 26. The method of claim 25, wherein the cells are transformed by electroporation, microporation or by particle bombardment. Claim: 27. The method of claim 26, wherein the RNAi-construct comprises SEQ ID NO: 1. Current U.S. Class: 435/134 Current International Class: 12; 12; 12; 12; 12; 12

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Decreasing RUBISCO content of algae and cyanobacteria cultivated in high carbon dioxide