Solar Urticaria : Basophil Activation Test With Uv-Irradiated Serum
Morressier, 2017
Online
unknown
Zugriff:
IntroductionAs underlying mechanism of solar urticaria, it is supposed that an inactive photoallergen in the skin or serum of patients with solar urticaria (SU) exists. This photoallergen is activated by irradiation, resulting in an immediate type hypersensitivity reaction with urticarial symptoms after sun exposure.1We wanted to investigate the existence of such a reactive photoallergen in the pathogenesis of solar urticaria, based on measuring the basophil activation with UV-irradiated serum of solar urticaria-patients and controls. Materials and MethodsBlood and serum were taken from three young men (18, 21 and 21 years) with solar urticaria, proven by phototesting (Tab. 1.). Blood and irradiated serum from individuals without SU were used as controls.Basophil activation tests (Flow CASTu00ae, Bu00fchlmann Laboratories AG) were performed to measure the allergen-specific response with UVA (50, 100 J/cmu00b2)and UVB (100, 200 mJ/cmu00b2) irradiated and unirradiated serum of patients and controls (Fig. 1.). Results: There was no significant CD63 activation of patients` or controls` basophils with the irradiated SU-patients` or controls` serum. Discussion: Despite positive results in a former case report 2 (CD203c expression was up-regulated more than 10% in relation to the negative control after irradiation with UVA 40 J/cm2) we were not able to activate basophils of our patients with SU by irradiated serum. We draw the conclusion that a photoallergen could not be detected in our patients by the method used. It is supposed, that there are two types of solar urticaria. In type I there is a pathological photoallergen in the patientsu2019 serum and in type II a precursor of the photoallergen in the patientsu2019 skin assumed.1 In our collective it is most likely, that the photoallergen is present in patients` skin, but not in serum. This explains the different outcome of studies with similar tests. Another cause could be that CD63 activation is not as sensitive as CD203c activation of basophils. It is also known that the photoallergen can differ in size and shape, which would explain the individual reaction and outcome. So more detailed information on fundamental pathophysiology of solar urticaria is needed to understand and characterise this complex disease completely.
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Solar Urticaria : Basophil Activation Test With Uv-Irradiated Serum
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Autor/in / Beteiligte Person: | Pietsch, Gunilla |
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Veröffentlichung: | Morressier, 2017 |
Medientyp: | unknown |
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