Pre-clinical outcome of alexidine-treated intravenous catheter for antimicrobial and antithrombogenic effectiveness
Morressier, 2017
Online
unknown
Zugriff:
Introduction:Intravenous catheters (IVCs) have advanced in recent years by incorporating antimicrobials such as silver and chlorhexidine, heparin etc. for minimizing catheter-related complications such as infection, occlusion and thrombosis. The purpose of this investigation was to assess antimicrobial and antithrombogenic effectiveness of a new IVC treated with Alexidine, an antimicrobial agent, in a sheep model with Staphylococcus aureus (SA) infection. Methods:Untreated (Control) and Alexidine-treated (ALX) IVCs were placed into 12 sheep for either 7- or 30-days (n=3 per group per timepoint) through left jugular vein in to superior-vena-cava. Prior to placement, insertion sites were inoculated with SA. Subsequently, explanted catheter, fibrin sheath and vein tissue were analyzed for microbial presence (via viable cell counts), fibrin sheath (FS) accumulation (via weight and length measurements) and venous thrombosis (by grading histopathological features). Additionally, clinical observations (such as body temperature, weight, blood cultures) were made through-out the study period. Results:Viable cell counts from the explanted catheters, sheath and surrounding tissues ranged between 10^5-10^8 CFU/cm from the 6 Control animals and 0 CFU/cm from the 6 ALX animals in both time cohorts. In the 30-day cohort, 2/3 Control animals also developed fever accompanied with SA positive blood cultures, while the 3 ALX animals remained healthy. In both time cohorts, all Control animals exhibited presence of extended FS, phlebitis, thickened vein wall, and complete venous occlusion in 3/6 animals; all ALX animals showed normal vein wall and FS presence limited at the insertion site. As compared to Control group, reduction in FS weight for the ALX group was 95% at 7-day and 99% at 30-day timepoints, while reduction in FS length was 82% at 7-day and 92% at 30-day timepoints.Conclusions:Alexidine-treated IVC was highly efficacious in reducing microbial colonization, fibrin sheath formation, CRBSI and venous thrombosis in the sheep model with SA infection.
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Pre-clinical outcome of alexidine-treated intravenous catheter for antimicrobial and antithrombogenic effectiveness
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Veröffentlichung: | Morressier, 2017 |
Medientyp: | unknown |
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