Molecular characterization of circulating Salmonella Typhi strains in an urban informal settlement in Kenya.
In: PLoS Neglected Tropical Diseases, Jg. 16 (2022-08-01), Heft 8, S. e0010704
Online
academicJournal
Zugriff:
A high burden of Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) bacteremia has been reported from urban informal settlements in sub-Saharan Africa, yet little is known about the introduction of these strains to the region. Understanding regional differences in the predominant strains of S. Typhi can provide insight into the genomic epidemiology. We genetically characterized 310 S. Typhi isolates from typhoid fever surveillance conducted over a 12-year period (2007-2019) in Kibera, an urban informal settlement in Nairobi, Kenya, to assess the circulating strains, their antimicrobial resistance attributes, and how they relate to global S. Typhi isolates. Whole genome multi-locus sequence typing (wgMLST) identified 4 clades, with up to 303 pairwise allelic differences. The identified genotypes correlated with wgMLST clades. The predominant clade contained 290 (93.5%) isolates with a median of 14 allele differences (range 0-52) and consisted entirely of genotypes 4.3.1.1 and 4.3.1.2. Resistance determinants were identified exclusively in the predominant clade. Determinants associated with resistance to aminoglycosides were observed in 245 isolates (79.0%), sulphonamide in 243 isolates (78.4%), trimethoprim in 247 isolates (79.7%), tetracycline in 224 isolates (72.3%), chloramphenicol in 247 isolates (79.6%), β-lactams in 239 isolates (77.1%) and quinolones in 62 isolates (20.0%). Multidrug resistance (MDR) determinants (defined as determinants conferring resistance to ampicillin, chloramphenicol and cotrimoxazole) were found in 235 (75.8%) isolates. The prevalence of MDR associated genes was similar throughout the study period (2007-2012: 203, 76.3% vs 2013-2019: 32, 72.7%; Fisher's Exact Test: P = 0.5478, while the proportion of isolates harboring quinolone resistance determinants increased (2007-2012: 42, 15.8% and 2013-2019: 20, 45.5%; Fisher's Exact Test: P
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Molecular characterization of circulating Salmonella Typhi strains in an urban informal settlement in Kenya.
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Autor/in / Beteiligte Person: | Ochieng, Caroline ; Jessica C Chen ; Mike Powel Osita ; Lee S Katz ; Griswold, Taylor ; Omballa, Victor ; Ng'eno, Eric ; Ouma, Alice ; Wamola, Newton ; Opiyo, Christine ; Achieng, Loicer ; Patrick K Munywoki ; Rene S Hendriksen ; Freeman, Molly ; Mikoleit, Matthew ; Juma, Bonventure ; Bigogo, Godfrey ; Mintz, Eric ; Jennifer R Verani ; Hunsperger, Elizabeth ; Heather A Carleton |
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Zeitschrift: | PLoS Neglected Tropical Diseases, Jg. 16 (2022-08-01), Heft 8, S. e0010704 |
Veröffentlichung: | Public Library of Science (PLoS), 2022 |
Medientyp: | academicJournal |
ISSN: | 1935-2727 (print) ; 1935-2735 (print) |
DOI: | 10.1371/journal.pntd.0010704 |
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