Molecular characterization of group A rotaviruses in Mukuru slums Kenya: detection of novel strains circulating in children below 5 years of age
In: BMC Research Notes, Jg. 10 (2017), Heft 1, S. 1-8
Online
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Abstract Background Gastroenteritis is a public health concern due to high morbidity and mortality among children. Rotaviruses are the leading etiological agents of severe gastroenteritis in children and accounts for more than half a million deaths per year in Africa. The study aimed at investigating the rotavirus genotypes that were circulating in children aged 5 years and below in and around Mukuru slums in Nairobi County Kenya. Methods A purposive cross sectional sampling method was applied where 166 samples were collected from children below 5 years of age and taken to Kenya Medical Research Institute virology laboratory. Presence of rotaviruses was determined using reverse transcription polymerase chain reaction, while extraction was done using ZR Soil/Fecal RNA MicroPrep™ extraction kit. This was followed by reverse transcription and genotyping using various group A rotavirus primers. Results The G type was successfully determined in 37 (92.5%), while the P type was successfully determined in 35 (87.5%) of the 40 (24%) page positive samples. Type G1 was the most predominant of the G types (40.5%), and the incidences of G3 and G9 were 21.6 and 32.4% respectively. Mixed types G3/G9 were detected at 5.4%. Three P types existed in Mukuru slums, P[8] (60%), P[6] (22.9%), P[4] (11.4) and their relative incidence varied over the 15 months of this study. Conclusions The G types and P types detected in this study are important causes of acute gastroenteritis in Mukuru slums Nairobi Kenya. An indication that the prevalence of certain genotypes may change over a rotavirus season is significant and mirrors observations from studies in other tropical climates. Thus monitoring of the genotypic changes among circulating viruses should be encouraged over the coming years.
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Molecular characterization of group A rotaviruses in Mukuru slums Kenya: detection of novel strains circulating in children below 5 years of age
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Autor/in / Beteiligte Person: | Joshua Ndung’u Gikonyo ; Nyangao, James ; Mbae, Cecilia ; Sang, Carlene ; Njagi, Eliud ; Ngeranwa, Joseph ; Esona, Mathew ; Seheri, Mapaseka L. ; Gitau, Grace W. ; Raini, Kedra ; Kariuki, Samuel |
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Zeitschrift: | BMC Research Notes, Jg. 10 (2017), Heft 1, S. 1-8 |
Veröffentlichung: | BMC, 2017 |
Medientyp: | academicJournal |
ISSN: | 1756-0500 (print) |
DOI: | 10.1186/s13104-017-2611-z |
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