The secreted PAMP-induced peptide StPIP1_1 activates immune responses in potato
In: Scientific Reports, Jg. 13 (2023), Heft 1, S. 1-12
Online
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Zugriff:
Abstract Treatment of potato plants with the pathogen-associated molecular pattern Pep-13 leads to the activation of more than 1200 genes. One of these, StPIP1_1, encodes a protein of 76 amino acids with sequence homology to PAMP-induced secreted peptides (PIPs) from Arabidopsis thaliana. Expression of StPIP1_1 is also induced in response to infection with Phytophthora infestans, the causal agent of late blight disease. Apoplastic localization of StPIP1_1-mCherry fusion proteins is dependent on the presence of the predicted signal peptide. A synthetic peptide corresponding to the last 13 amino acids of StPIP1_1 elicits the expression of the StPIP1_1 gene itself, as well as that of pathogenesis related genes. The oxidative burst induced by exogenously applied StPIP1_1 peptide in potato leaf disks is dependent on functional StSERK3A/B, suggesting that StPIP1_1 perception occurs via a receptor complex involving the co-receptor StSERK3A/B. Moreover, StPIP1_1 induces expression of FRK1 in Arabidopsis in an RLK7-dependent manner. Expression of an RLK from potato with high sequence homology to AtRLK7 is induced by StPIP1_1, by Pep-13 and in response to infection with P. infestans. These observations are consistent with the hypothesis that, upon secretion, StPIP1_1 acts as an endogenous peptide required for amplification of the defense response.
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The secreted PAMP-induced peptide StPIP1_1 activates immune responses in potato
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Autor/in / Beteiligte Person: | Nietzschmann, Linda ; Smolka, Ulrike ; Elvio Henrique Benatto Perino ; Gorzolka, Karin ; Stamm, Gina ; Marillonnet, Sylvestre ; Bürstenbinder, Katharina ; Rosahl, Sabine |
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Zeitschrift: | Scientific Reports, Jg. 13 (2023), Heft 1, S. 1-12 |
Veröffentlichung: | Nature Portfolio, 2023 |
Medientyp: | academicJournal |
ISSN: | 2045-2322 (print) |
DOI: | 10.1038/s41598-023-47648-x |
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