Entire Expressed Peripheral Blood Transcriptome in Pediatric Severe Malarial Anemia
In: Res Sq, 2023
academicJournal
Zugriff:
This study on severe malarial anemia (SMA: Hb < 6.0 g/dL), a leading global cause of childhood morbidity and mortality, analyzed the entire expressed transcriptome in whole blood from children with non-SMA (Hb ≥ 6.0 g/dL, n = 41) and SMA (n = 25). Analyses revealed 3,420 up-regulated and 3,442 down-regulated transcripts, signifying impairments in host inflammasome activation, cell death, innate immune responses, and cellular stress responses in SMA. Immune cell profiling showed a decreased antigenic and immune priming response in children with SMA, favoring polarization toward cellular proliferation and repair. Enrichment analysis further identified altered neutrophil and autophagy-related processes, consistent with neutrophil degranulation and altered ubiquitination and proteasome degradation. Pathway analyses highlighted SMA-related alterations in cellular homeostasis, signaling, response to environmental cues, and cellular and immune stress responses. Validation with a qRT-PCR array showed strong concordance with the sequencing data. These findings identify key molecular themes in SMA pathogenesis, providing potential targets for new malaria therapies.
Titel: |
Entire Expressed Peripheral Blood Transcriptome in Pediatric Severe Malarial Anemia
|
---|---|
Autor/in / Beteiligte Person: | Anyona, Samuel ; Cheng, Qiuying ; Guo, Yan ; Raballah, Evans ; Hurwitz, Ivy ; Onyango, Clinton ; Seidenberg, Philip ; Schneider, Kristan ; Lambert, Christophe ; McMahon, Benjamin ; Ouma, Collins ; Perkins, Douglas |
Link: | |
Zeitschrift: | Res Sq, 2023 |
Veröffentlichung: | American Journal Experts, 2023 |
Medientyp: | academicJournal |
DOI: | 10.21203/rs.3.rs-3150748/v1 |
Schlagwort: |
|
Sonstiges: |
|