High resolution MALDI-TOF-MS and MS/MS: Application for the structural characterization of sulfated oligosaccharides
In: European Journal of Mass Spectrometry, Jg. 25 (2019), Heft 5, S. 428-436
academicJournal
Zugriff:
Sulfated oligosaccharides are involved in important biological events that are often modulated by specific sequences and sulfation patterns, but their structural analysis remains challenging. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) analysis of three different sulfated oligosaccharides (Fondaparinux, the octasulfated pentasaccharide, a disulfated heparin-derived tetrasaccharide 1, and an octasulfated maltoheptaose) 2 was performed using the 2-(4-hydroxyphenylazo)benzoic acid-tetramethylguanidinium (HABA-TMG 2 ) matrix. High resolution mass spectrometry of the main ions observed was successful, and this was complemented by tandem mass spectrometry (MS/MS) analysis for structural assessment. Despite sulfate losses, fully sulfated molecular ions were observed and these allowed the determination of oligosaccharide structures: UA-GlcNAc-UA(2S)-AnhMan(6S) for compound 1 and (Glc6S) 6 -Glc (1S,6S) for compound 2.
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High resolution MALDI-TOF-MS and MS/MS: Application for the structural characterization of sulfated oligosaccharides
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Autor/in / Beteiligte Person: | Lesur, David ; Duhirwe, Gilbert ; Kovensky, José ; Centre National de la Recherche Scientifique ; the Ministére de l'Enseignement Supérieur et de la Recherche |
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Zeitschrift: | European Journal of Mass Spectrometry, Jg. 25 (2019), Heft 5, S. 428-436 |
Veröffentlichung: | SAGE Publications, 2019 |
Medientyp: | academicJournal |
ISSN: | 1469-0667 |
DOI: | 10.1177/1469066719851438 |
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