Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
In: ISSN: 2331-8325 ; Bio-protocol ; https://hal.archives-ouvertes.fr/hal-03211272, 2018
academicJournal
Zugriff:
International audience ; Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and characterization of resistance sources. This paper describes a protocol for mechanical inoculation of rice seedlings with RYMV and two methods of resistance evaluation - one based on a symptom severity index and the other on virus detection through double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA).
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Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
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Autor/in / Beteiligte Person: | Pinel-Galzi, Agnès ; Hébrard, Eugénie ; Traoré, Oumar ; Silué, Drissa ; Albar, Laurence ; Institut de Recherche pour le Développement (IRD) ; Institut de l'Environnement et Recherches Agricoles Ouagadougou (INERA) ; Diversité, adaptation, développement des plantes (UMR DIADE) ; Institut de Recherche pour le Développement (IRD France-Sud )-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
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Zeitschrift: | ISSN: 2331-8325 ; Bio-protocol ; https://hal.archives-ouvertes.fr/hal-03211272, 2018 |
Veröffentlichung: | HAL CCSD ; Bio-protocol LCC, 2018 |
Medientyp: | academicJournal |
DOI: | 10.21769/BioProtoc.2863 |
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