Serology- and Blood-PCR-Based Screening for Schistosomiasis in Pregnant Women in Madagascar—A Cross-Sectional Study and Test Comparison Approach
In: Pathogens, Vol 10, Iss 722, p 722 (2021, Jg. 10 (2021), Heft 722, p 722
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Zugriff:
This work was conducted as a cross sectional study to define the disease burden of schistosomiasis in pregnant Madagascan women and to evaluate serological and molecular diagnostic assays. A total of 1154 residual EDTA blood samples from pregnant Madagascan women were assessed. The nucleic acid extractions were subjected to in-house real-time PCRs specifically targeting S. mansoni complex, S. haematobium complex, and African Schistosoma spp. on genus level, while the EDTA plasma samples were analyzed using Schistosoma -specific IgG and IgM commercial ELISA and immunofluorescence assays. The analyses indicated an overall prevalence of schistosomiasis in Madagascan pregnant women of 40.4%, with only minor regional differences and differences between serology- and blood PCR-based surveillance. The S. mansoni specific real-time PCR showed superior sensitivity of 74% (specificity 80%) compared with the genus-specific real-time PCR (sensitivity 13%, specificity 100%) in blood. The laborious immunofluorescence (sensitivity IgM 49%, IgG 87%, specificity IgM 85%, IgG 96%) scored only slightly better than the automatable ELISA (sensitivity IgM 38%, IgG 88%, specificity IgM 78%, IgG 91%). Infections with S. mansoni were detected only. The high prevalence of schistosomiasis recorded here among pregnant women in Madagascar calls for actions in order to reduce the disease burden.
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Serology- and Blood-PCR-Based Screening for Schistosomiasis in Pregnant Women in Madagascar—A Cross-Sectional Study and Test Comparison Approach
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Autor/in / Beteiligte Person: | Hoffmann, Tanja ; Carsjens, Imke ; Rakotozandrindrainy, Raphaël ; Girmann, Mirko ; Randriamampionona, Njary ; Maïga-Ascofaré, Oumou ; Podbielski, Andreas ; Hahn, Andreas ; Frickmann, Hagen ; Norbert Georg Schwarz |
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Zeitschrift: | Pathogens, Vol 10, Iss 722, p 722 (2021, Jg. 10 (2021), Heft 722, p 722 |
Veröffentlichung: | MDPI AG, 2021 |
Medientyp: | academicJournal |
ISSN: | 2076-0817 (print) |
DOI: | 10.3390/pathogens10060722 |
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