Distinct lineages of Ebola virus in Guinea during the 2014 West African epidemic
In: ISSN: 0028-0836, 2015
Online
academicJournal
Zugriff:
International audience ; An epidemic of Ebola virus disease of unprecedented scale has been ongoing for more than a year in West Africa. As of 29 April 2015, there have been 26,277 reported total cases (of which 14,895 have been laboratory confirmed) resulting in 10,899 deaths. The source of the outbreak was traced to the prefecture of Guéckédou in the forested region of southeastern Guinea. The virus later spread to the capital, Conakry, and to the neighbouring countries of Sierra Leone, Liberia, Nigeria, Senegal and Mali. In March 2014, when the first cases were detected in Conakry, the Institut Pasteur of Dakar, Senegal, deployed a mobile laboratory in Donka hospital to provide diagnostic services to the greater Conakry urban area and other regions of Guinea. Through this process we sampled 85 Ebola viruses (EBOV) from patients infected from July to November 2014, and report their full genome sequences here. Phylogenetic analysis reveals the sustained transmission of three distinct viral lineages co-circulating in Guinea, including the urban setting of Conakry and its surroundings. One lineage is unique to Guinea and closely related to the earliest sampled viruses of the epidemic. A second lineage contains viruses probably reintroduced from neighbouring Sierra Leone on multiple occasions, while a third lineage later spread from Guinea to Mali. Each lineage is defined by multiple mutations, including non-synonymous changes in the virion protein 35 (VP35), glycoprotein (GP) and RNA-dependent RNA polymerase (L) proteins. The viral GP is characterized by a glycosylation site modification and mutations in the mucin-like domain that could modify the outer shape of the virion. These data illustrate the ongoing ability of EBOV to develop lineage-specific and potentially phenotypically important variation.
Titel: |
Distinct lineages of Ebola virus in Guinea during the 2014 West African epidemic
|
---|---|
Autor/in / Beteiligte Person: | Simon-Loriere, Etienne ; Faye, Ousmane ; Faye, Oumar ; Koivogui, Lamine ; Magassouba, Nfaly ; Keita, Sakoba ; Thiberge, Jean-Michel ; Diancourt, Laure ; Bouchier, Christiane ; Vandenbogaert, Matthias ; Caro, Valérie ; Fall, Gamou ; Buchmann, Jan ; Matranga, Christan ; Sabeti, Pardis ; Manuguerra, Jean-Claude ; Holmes, Edward ; Sall, Amadou ; Génétique fonctionnelle des Maladies infectieuses - Functional Genetics of Infectious Diseases ; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS) ; Institut Pasteur de Dakar ; Réseau International des Instituts Pasteur (RIIP) ; Arbovirus et Virus de Fièvres Hémorragiques Dakar, Sénégal ; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) ; Institut National de Santé Publique Conakry, Guinée (INSP) ; Ministère de la Santé Conakry, Guinea ; Université Gamal Abdel Nasser de Conakry ; Ministry of Health Guinée ; Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU) ; Institut Pasteur Paris (IP) ; Environnement et Risques infectieux - Environment and Infectious Risks (ERI) ; Génomique (Plate-Forme) - Genomics Platform ; Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8) ; The University of Sydney ; Broad Institute Cambridge ; Harvard University-Massachusetts Institute of Technology (MIT) ; University, Harvard ; This study was supported by the Pasteur Ebola Task Force (PETF) and has also received funding from the French government’s Investissement d’Avenir programme, Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (grant number ANR-10-LABX-62-IBEID) and Institut Pasteur de Dakar. We are grateful to all members of the PETF for their support, and in particular F. Rey and K. Victoir. High-throughput sequencing was performed on the Genomics Platform of Institut Pasteur, member of ‘France Génomique’ consortium (ANR10-INBS-09-08). ; We thank L. Ma for technical assistance and Institut Pasteur Clinical Research Department for their help with ethical approval procedures. E.C.H. is supported by an NHMRC Australia fellowship. ; ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010) ; ANR-10-INBS-0009,France Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010) |
Link: | |
Zeitschrift: | ISSN: 0028-0836, 2015 |
Veröffentlichung: | HAL CCSD ; Nature Publishing Group, 2015 |
Medientyp: | academicJournal |
DOI: | 10.1038/nature14612 |
Schlagwort: |
|
Sonstiges: |
|