Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon ; Lokalizacija auksina i proteina PIN tijekom somatske embriogeneze vrste Brachypodium distachyon

Brachypodium distachyon is a model species used for research of Poaceae. The phylogenetic link this species shares with monocots of agronomic importance makes it the ideal model for studying developmental mechanism with economic applications, like somatic embryogenesis (SE). As auxin plays a critical role in SE, tracking its presence during SE enables description of the process as a whole. For this purpose, fluorescently-tagged PIN-FORMED (PIN) auxin transporters and presence of auxin was examined using confocal microscopy. Findings confirmed PIN1a to be a major marker of SE as it is expressed in all stages of SE and revealed the complementary but mutually exclusive distribution patterns of SoPIN1 and PIN1b. Moreover, examinations of markers of auxin presence revealed how SoPIN1 and PIN1b affect its flow and thus influence SE. Additionally, with the goal of furthering understanding of the effect these select PIN auxin transporters have on SE, response of loss-of-function mutants pin1a, pin1b and their cross pin1a × pin1b to in vitro SE induction was compared using PAS/NBB staining. Results have shown no identifiable histological differences in comparison to wild-type explants, hinting at functional redundancy of the remaining PIN transporters allowing SE to occur. ; Brachypodium distachyon je vrsta koja se koristi pri proučavanju porodice Poaceae. Njena filogenetska povezanost s jednosupnicama od agronomske važnosti čini je idealnom za proučavanje razvojnih mehanizama s ekonomskim primjenama, poput somatske embriogeneze (SE). Kako auksin ima ključnu ulogu u SE, praćenje njegove prisutnosti tijekom SE omogućava opisivanje tog procesa u cjelini. U tu svrhu, odabrani su fluorescentno označeni transporteri auksina PIN-FORMED (PIN) za prisutnost auksina te proučeni pomoću konfokalnog mikroskopa ili konfokalne mikroskopije. Rezultati su pokazali da je PIN1a važan marker SE budući da je eksprimiran tijekom svih stadija SE te da su obrasci distribucije SoPIN1 i PIN1b komplementarni i međusobno isključivi. Štoviše, ...