Locoregional intravenous delivery of AAV vector in macaque results in persistent transgene expression despite a dedicated humoral and cellular immune response
In: 16th annual meeting ; https://hal.science/hal-01190314 ; 16th annual meeting, May 2013, Salt Lake City, United States. 2013, 2013
Online
Konferenz
Zugriff:
After LR delivery of an AAV8 vector expressing GFP in non-human primates, we showed detection of transgene expression until at least 8 months post-injection in contrast to IM where GFP was lost at 3 months post-injection. Interestingly, GFP persistence after LR was observed despite the detection of anti-GFP IgG antibodies, even at higher titers than IM route, and the presence of T cells responding to GFP peptides in the spleen. Surprisingly, GFP-restimulated T cells secreted IFNγ but not IL2 after LR as compared to IM route where both cytokines were detected.
| Titel: |
Locoregional intravenous delivery of AAV vector in macaque results in persistent transgene expression despite a dedicated humoral and cellular immune response
|
|---|---|
| Autor/in / Beteiligte Person: | Gernoux, Gwaldys ; Devaux, Marie ; Dubreil, Laurence ; Jaulin, Nicolas ; Guilbaud, Mickaël ; Deschamps, Jack-Yves ; Larcher, Thibaut ; Guigand, Lydie ; Dutilleul, Maeva ; Le Guiner, Caroline ; Cherel, Yan ; Mouillier, Philippe ; Adjali, Oumeya ; Laboratoire de Thérapie Génique Translationnelle des Maladies Génétiques (Inserm UMR 1089) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE) ; Université de Nantes (UN)-Université de Nantes (UN) ; Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; University of Florida Gainesville (UF) ; American Society of Cell and Gene Therapy (ASGCT). USA. |
| Link: | |
| Zeitschrift: | 16th annual meeting ; https://hal.science/hal-01190314 ; 16th annual meeting, May 2013, Salt Lake City, United States. 2013, 2013 |
| Veröffentlichung: | HAL CCSD, 2013 |
| Medientyp: | Konferenz |
| Schlagwort: |
|
| Sonstiges: |
|