Carriage of ESBL-producing Klebsiella pneumoniae and Escherichia coli among children in rural Ghana: a cross-sectional study
In: Antimicrobial Resistance and Infection Control, Jg. 12 (2023), Heft 1, S. 1-9
Online
academicJournal
Zugriff:
Background Extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae (ESBL-KP) and Escherichia coli (ESBL-EC) present a high burden in both communities and healthcare sectors, leading to difficult-to-treat infections. Data on intestinal carriage of ESBL-KP and ESBL-EC in children is scarce, especially in sub-Saharan African countries. We provide data on faecal carriage, phenotypic resistance patterns, and gene variation of ESBL-EC and ESBL-KP among children in the Agogo region of Ghana. Methods From July to December 2019, fresh stool samples were collected within 24 h from children < 5 years with and without diarrhoea attending the study hospital. The samples were screened for ESBL-EC and ESBL-KP on ESBL agar and confirmed using double-disk synergy testing. Bacterial identification and an antibiotic susceptibility profile were performed using the Vitek 2 compact system (bioMérieux, Inc.). ESBL genes, blaSHV, blaCTX-M, and blaTEM were identified by PCR and further sequencing. Results Of the 435 children recruited, stool carriage of ESBL-EC and ESBL-KP was 40.9% (n/N = 178/435) with no significant difference in prevalence between children with diarrhoea and non-diarrhoea. No association between ESBL carriage and the age of the children was found. All isolates were resistant to ampicillin and susceptible to meropenem and imipenem. Both ESBL-EC and ESBL-KP isolates showed over 70% resistance to tetracycline and sulfamethoxazole-trimethoprim. Multidrug resistance was observed in over 70% in both ESBL-EC and ESBL-KP isolates. The blaCTX-M-15 was the most prevalent ESBL gene detected. blaCTX-M-27, blaCTX-M-14, and blaCTX-M-14b were found in non-diarrhoea stools of children, whereas blaCTX-M-28 was found in both the diarrhoea and non-diarrhoea patient groups. Conclusions The carriage of ESBL-EC and ESBL-KP among children with and without diarrhoea in the Agogo community with a high prevalence of blaCTX-M-15 is noteworthy, highlighting the importance of both the population as a possible ...
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Carriage of ESBL-producing Klebsiella pneumoniae and Escherichia coli among children in rural Ghana: a cross-sectional study
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Autor/in / Beteiligte Person: | Charity Wiafe Akenten ; Neyaz Ahmed Khan ; Mbwana, Joyce ; Krumkamp, Ralf ; Fosu, Dennis ; Ellis Kobina Paintsil ; Kennedy Gyau Boahen ; Osei-Mensa, James ; Maiga-Ascofare, Oumou ; May, Jürgen ; Obiri-Danso, Kwasi ; Richard Odame Phillips ; Linda Aurelia Ofori ; Dekker, Denise |
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Zeitschrift: | Antimicrobial Resistance and Infection Control, Jg. 12 (2023), Heft 1, S. 1-9 |
Veröffentlichung: | BMC, 2023 |
Medientyp: | academicJournal |
ISSN: | 2047-2994 (print) |
DOI: | 10.1186/s13756-023-01263-7 |
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