β-D-phosphogalactoside galactohydrolase of Streptococcus faecalis and the inhibition of its synthesis by glucose
In: Canadian Journal of Microbiology, Jg. 24 (1978), Heft 5, S. 512-519
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Zugriff:
The lactose hydrolysing system of Streptococcus faecalis is described. It is closely related to that one of the group N streptococci as it consists of a β-D-phosphogalactoside galactohydrolase β-Pgal). The uptake of methyl-β-D-thiogalactoside (TMG), lactose, and glucose is maintained by the phosphoenolpyruvate-dependent phosphotransferase system (PTS) but the uptake of galactose is not. The induction time is 6–7 min. Inducers are lactose and galactose but not isopropyl-β-D-galactoside (IPTG) and TMG. In the presence of glucose, mannose, and maltose no induction of β-Pgal occurs but pyruvate and glycerol allow induction. The competitive inhibition of uptake of TMG by glucose suggests inducer exclusion by this sugar. TMG accumulates in the cells exclusively as a derivative.
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β-D-phosphogalactoside galactohydrolase of Streptococcus faecalis and the inhibition of its synthesis by glucose
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Autor/in / Beteiligte Person: | Heller, K. ; Röschenthaler, R. |
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Zeitschrift: | Canadian Journal of Microbiology, Jg. 24 (1978), Heft 5, S. 512-519 |
Veröffentlichung: | Canadian Science Publishing, 1978 |
Medientyp: | academicJournal |
ISSN: | 0008-4166 |
DOI: | 10.1139/m78-084 |
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