Cleavage at the twelve-base-pair sequence 5'-TCTAGATCTAGA-3' using M{middle dot}Xbal (TCTAGm6A) methylation and Dpnl (Gm6A/TC) cleavage
Oxford University Press, 1990
academicJournal
Zugriff:
The DNA methylase M· Xbal was isolated from an E. coli recombinant clone. We deduce that the enzyme methylates at the sequence 5′-TCTAGm6A-3′. In combination with the methyiation-dependent restriction endonuclease, Dpnl (5′-Gm6A/TC-3′), DNA cleavage occurs at the sequence 5′-TCTAGA/TCTAGA-3′. This twelve-base-pair site should occur once every 16,000,000 base pairs in a random sequence of DNA. The exceptional rarity of the M· Xbal / Dpnl sequence makes it an ideal candidate for transpositional integration of a unique cleavage site into bacterial genomes. Retrotransposition into mammalian genomes is also an attractive possibility.
Titel: |
Cleavage at the twelve-base-pair sequence 5'-TCTAGATCTAGA-3' using M{middle dot}Xbal (TCTAGm6A) methylation and Dpnl (Gm6A/TC) cleavage
|
---|---|
Autor/in / Beteiligte Person: | Patel, Yogesh ; Van Cott, Elizabeth ; Wilson, Geoffrey G. ; McClelland, Michael |
Link: | |
Veröffentlichung: | Oxford University Press, 1990 |
Medientyp: | academicJournal |
DOI: | 10.1093/nar/18.6.1603 |
Schlagwort: |
|
Sonstiges: |
|