Molecular characterization of vancomycin-resistant enterococci and extended-spectrum beta-lactamase-containing Escherichia coli isolates in wild birds from the Azores Archipelago
In: N (eISSN); 0307-9457 (ISSN); CAVP-2011-0044 (PII); CAVP-2011-0044.R1 (manuscript); CAVP-2011-0044.R1 (publisherID); Avian Pathology; 123; 18; 38; 13; 58; 50; 57; 118; 65; 14; 54; 33; 49; 66; 24; 67; 60; 23; 76; 59; 88; 55; 52; 53; 48; 9 Suppl 1; 27; 1-2; 47; 3-4; 10; 2-3; 12; 468; 2774; 1311; 139; 832; 299; 1939; 933; 969; 610; 16; 4555; 113; 523; 1628; 1133; 1137; 449; 8126; 165; 636; 291; 657; 4118; 278; 396; 203; 584; 171; 537; 3996; 215; 1306; 316; S47; 301; 472; 2777; 1312; 141; 839; 304; 1944; 51, table of contents; 976; 617; 4558; 116; 531; 1635; 1136; 1141; 454; 8134; 174; 638; 686; 4120; 280; 402; 208; 588; 173; 541; 4001; 224; 1307; 319; S52; 305; International Journal of Food Microbiology (); Journal of Chemotherapy (); Journal of Clinical Microbiology (); Euro Surveillance (); Journal of Antimicrobial Chemotherapy (); CLSI document M31-A3. CLSI, Wayne, PA, U.S.A., (); Avian Diseases (); J Antimicrob Chemother (); Veterinary Microbiology (); Clinical Microbiology Reviews (); Antimicrobial Agents and Chemotherapy (); Avian Pathology (); Appl Environ Microbiol (); J Assoc Avian Vet (); Avian Pathol (); Applied and Environmental Microbiology (); Foodborne Pathogens and Disease (); Oral Microbiology and Immunology (); Emerging Infectious Diseases (); Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health (); Journal of Basic Microbiology (); Letters in Applied Microbiology (); Proteome Science (); Microbes Infect (); Clin Microbiol Infect (); Microbial Drug Resistance (); Indian Journal of Medical Microbiology (); (2012)
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Zugriff:
To study the prevalence of vancomycin-resistant enterococci (VRE) and extended-spectrum beta-lactamases (ESBLs) containing Escherichia coli isolates, and the mechanisms of resistance implicated, 220 faecal samples from wild birds were collected between 2006 and 2010 in the Azores Archipelago. Samples were spread in Slanetz-Bartley agar plates supplemented with 4 mg/L of vancomycin and in Levine agar plates supplemented with 2 mg/L of cefotaxime for VRE and ESBL-containing E. coli isolation, respectively. vanA-containing enterococcal isolates (four Enterococcus faecium and two E. durans) and vanC-1 E. gallinarum isolates were detected in six and seven faecal samples, respectively. VRE isolates showed ampicillin (n= 11), ciprofloxacin (n= 9), tetracycline (n= 6), erythromycin (n= 5), quinupristin/dalfopristin (n= 3) and high-level kanamycin resistance (n= 1). The tet(L) and/or tet(M) gene, was found in all tetracycline-resistant isolates and the erm(B) gene in all erythromycin-resistant isolates. Three vanA-containing E. faecium and two E. gallinarum presented specific sequences of the Tn5397 transposon. Four VRE isolates harboured the ace virulence gene. One faecal sample reveals one ESBL-containing E. coli isolate that belongs to the A phylogenetic group, showed a phenotype of resistance to beta-lactams and tetracycline and harbored the blaCTX-M-14, blaSHV-12 and the tet(A) genes. To our knowledge, this is the first study to focus on defining the prevalence of VRE and/or ESBL-containing E. coli strains in wild birds from the Azores. The data recovered are essential to improve knowledge about the dissemination of resistant strains through wild ecosystems and their possible implications by transferring these resistances to other animals or to humans. ; nsilva@utad.pt (Silva, Nuno) ; gigrejas@utad.pt (Igrejas, Gilberto) ; pedroreisrodrigues@yahoo.com (Rodrigues, Pedro) ; tmgrodrigues@gmail.com (Rodrigues, Tiago) ; alexfgoncalves@gmail.com (Goncalves, Alexandre) ; carolinafelgar@gmail.com (Felgar, Ana) ; ruicruzp@gmail.com (Pacheco, Rui) ; drgoncal@fc.up.pt (Goncalves, David) ; rcunha@uac.pt (Cunha, Regina) ; ppoeta@utad.pt (Poeta, Patricia) ; University of Tras-os-Montes and Alto Douro, CECAV - Vila Real - PORTUGAL (Silva, Nuno) ; University of Tras-os-Montes and Alto Douro, Department of Genetics and Biotechnology - Vila Real - PORTUGAL (Igrejas, Gilberto) ; University of the Azores, Department of Biology - Ponta Delgada - PORTUGAL (Rodrigues, Pedro) ; University of Porto, CIBIO - Porto - PORTUGAL (Rodrigues, Tiago) ; University of Tras-os-Montes and Alto Douro, Department of Veterinary Sciences - Vila Real - PORTUGAL (Goncalves, Alexandre) ; University of Tras-os-Montes and Alto Douro, Department of Genetics and Biotechnology - Vila Real - PORTUGAL (Felgar, Ana) ; University of Tras-os-Montes and Alto Douro, Department of Veterinary Sciences - Vila Real - PORTUGAL (Pacheco, Rui) ; University of Porto, CIBIO - Porto - PORTUGAL (Goncalves, David) ; University of the Azores, Department of Biology - Ponta Delgada - PORTUGAL (Cunha, Regina) ; University of Tras-os-Montes and Alto Douro, Department of Veterinary Sciences - Quinta de Prados--> - 5000-801 - Vila Real - PORTUGAL (Poeta, Patricia) ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL ; PORTUGAL
| Titel: |
Molecular characterization of vancomycin-resistant enterococci and extended-spectrum beta-lactamase-containing Escherichia coli isolates in wild birds from the Azores Archipelago
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| Quelle: | N (eISSN); 0307-9457 (ISSN); CAVP-2011-0044 (PII); CAVP-2011-0044.R1 (manuscript); CAVP-2011-0044.R1 (publisherID); Avian Pathology; 123; 18; 38; 13; 58; 50; 57; 118; 65; 14; 54; 33; 49; 66; 24; 67; 60; 23; 76; 59; 88; 55; 52; 53; 48; 9 Suppl 1; 27; 1-2; 47; 3-4; 10; 2-3; 12; 468; 2774; 1311; 139; 832; 299; 1939; 933; 969; 610; 16; 4555; 113; 523; 1628; 1133; 1137; 449; 8126; 165; 636; 291; 657; 4118; 278; 396; 203; 584; 171; 537; 3996; 215; 1306; 316; S47; 301; 472; 2777; 1312; 141; 839; 304; 1944; 51, table of contents; 976; 617; 4558; 116; 531; 1635; 1136; 1141; 454; 8134; 174; 638; 686; 4120; 280; 402; 208; 588; 173; 541; 4001; 224; 1307; 319; S52; 305; International Journal of Food Microbiology (); Journal of Chemotherapy (); Journal of Clinical Microbiology (); Euro Surveillance (); Journal of Antimicrobial Chemotherapy (); CLSI document M31-A3. CLSI, Wayne, PA, U.S.A., (); Avian Diseases (); J Antimicrob Chemother (); Veterinary Microbiology (); Clinical Microbiology Reviews (); Antimicrobial Agents and Chemotherapy (); Avian Pathology (); Appl Environ Microbiol (); J Assoc Avian Vet (); Avian Pathol (); Applied and Environmental Microbiology (); Foodborne Pathogens and Disease (); Oral Microbiology and Immunology (); Emerging Infectious Diseases (); Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health (); Journal of Basic Microbiology (); Letters in Applied Microbiology (); Proteome Science (); Microbes Infect (); Clin Microbiol Infect (); Microbial Drug Resistance (); Indian Journal of Medical Microbiology (); (2012) |
| Veröffentlichung: | Taylor & Francis, 2012 |
| Medientyp: | unknown |
| DOI: | 10.1080/03079457.2011.599061 |
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