Extravascular dermal trypanosomes in suspected and confirmed cases of gambiense Human African Trypanosomiasis
In: ISSN: 1058-4838, 2021
Online
academicJournal
Zugriff:
International audience ; Background: The diagnosis of gambiense human African trypanosomiasis (gHAT) typically involves 2 steps: a serological screen, followed by the detection of living trypanosome parasites in the blood or lymph node aspirate. Live parasites can, however, remain undetected in some seropositive individuals, who, we hypothesize, are infected with Trypanosoma brucei gambiense parasites in their extravascular dermis.Methods: To test this hypothesis, we conducted a prospective observational cohort study in the gHAT focus of Forecariah, Republic of Guinea. Of the 5417 subjects serologically screened for gHAT, 66 were enrolled into our study and underwent a dermatological examination. At enrollment, 11 seronegative, 8 unconfirmed seropositive, and 18 confirmed seropositive individuals had blood samples and skin biopsies taken and examined for trypanosomes by molecular and immunohistological methods.Results: In seropositive individuals, dermatological symptoms were significantly more frequent, relative to seronegative controls. T.b. gambiense parasites were present in the blood of all confirmed cases (n = 18) but not in unconfirmed seropositive individuals (n = 8). However, T. brucei parasites were detected in the extravascular dermis of all unconfirmed seropositive individuals and all confirmed cases. Skin biopsies of all treated cases and most seropositive untreated individuals progressively became negative for trypanosomes 6 and 20 months later.Conclusions: Our results highlight the skin as a potential reservoir for African trypanosomes, with implications for our understanding of this disease's epidemiology in the context of its planned elimination and underlining the skin as a novel target for gHAT diagnostics.
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Extravascular dermal trypanosomes in suspected and confirmed cases of gambiense Human African Trypanosomiasis
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Autor/in / Beteiligte Person: | Camara, Mariame ; M'Mah Soumah, Alseny ; Ilbouldo, Hamidou ; Travaillé, Christelle ; Clucas, Caroline ; Cooper, Anneli ; Kuispond Swar, Nono-Raymond ; Camara, Oumou ; Sadissou, Ibrahim ; Calvo Alvarez, Estefania ; Crouzols, Aline ; Bart, Jean-Mathieu ; Jamonneau, Vincent ; Camara, Mamadou ; Macleod, Annette ; Bucheton, Bruno ; Rotureau, Brice ; Ministère de la Santé Conakry, Guinea ; Donka, Hôpital ; Institut de Recherche en Sciences de la Santé Ouagadougou, Burkina Faso (IRSS) ; Institut de Recherche pour le Développement (IRD) ; Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology ; Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) ; College of Medical, Veterinary and Life Sciences Glasgow ; University of Glasgow ; Institut National de Recherche Biomédicale Kinshasa (INRB) ; Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Université de Bordeaux (UB) ; This work was supported by the Wellcome Trust (209511/Z/17/Z), the Institut de Recherche pour le Développement, the Institut Pasteur, the French Government Investissement d'Avenir programme - Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases (ANR-10-LABX-62-IBEID) and the French National Agency for Scientific Research (projects ANR-14-CE14-0019-01 EnTrypa and ANR-18-CE15-0012 TrypaDerm). ; ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010) ; ANR-14-CE14-0019,ENTRYPA,Dynamique des étapes précoces de l'infection de l'hôte mammifère par les trypanosomes africains(2014) ; ANR-18-CE15-0012,TrypaDerm,Comprendre la biologie des trypanosomes africains dans la peau(2018) |
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Zeitschrift: | ISSN: 1058-4838, 2021 |
Veröffentlichung: | HAL CCSD ; Oxford University Press (OUP), 2021 |
Medientyp: | academicJournal |
DOI: | 10.1101/2020.02.24.20026211 |
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