A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus
In: ISSN: 1935-2727, 2016
academicJournal
Zugriff:
International audience ; BACKGROUND:Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis.METHODOLOGY/PRINCIPAL FINDINGS:In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%.CONCLUSIONS/SIGNIFICANCE:The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need.
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A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus
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Autor/in / Beteiligte Person: | Patel, Pranav ; Abd El Wahed, Ahmed ; Faye, Oumar ; Prüger, Pauline ; Kaiser, Marco ; Thaloengsok, Sasikanya ; Ubol, Sukathida ; Sakuntabhai, Anavaj ; Leparc-Goffart, Isabelle ; Hufert, Frank ; Sall, Amadou ; Weidmann, Manfred ; Niedrig, Matthias ; Robert Koch Institute Berlin (RKI) ; Göttingen, Georg-August-University ; Arbovirus et Virus de Fièvres Hémorragiques Dakar, Sénégal ; Institut Pasteur de Dakar ; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) ; GenExpress Gesellschaft für Proteindesign mbH ; Mahidol University Bangkok ; Génétique fonctionnelle des Maladies infectieuses - Functional Genetics of Infectious Diseases ; Institut Pasteur Paris -Centre National de la Recherche Scientifique (CNRS) ; Institut de Recherche Biomédicale des Armées Antenne Marseille (IRBA) ; Medizinische Hochschule Brandenburg Theodor Fontane / Brandenburg Medical School Theodor-Fontane (MHB Theodor Fontane) ; University of Stirling ; The authors received no specific funding for this work. |
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Zeitschrift: | ISSN: 1935-2727, 2016 |
Veröffentlichung: | HAL CCSD ; Public Library of Science, 2016 |
Medientyp: | academicJournal |
DOI: | 10.1371/journal.pntd.0004953 |
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