Immunoprecipitation and Western blot-based detection of protein O-GlcNAcylation in cells
In: STAR Protocols, Jg. 3 (2022), Heft 1, S. 101108-
Online
academicJournal
Zugriff:
Summary: Detection of protein O-GlcNAcylation could be challenging. By using the host-cell factor 1 (HCF-1), a known O-GlcNAcylated protein, we immunoprecipitated HCF-1 from transfected HEK293T cells or endogenous HCF-1 from HeLa cells to detect its O-GlcNAc levels by Western blotting. We also take advantage of RNAi or chemical inhibitors to modulate OGT and OGA activities before HCF-1 immunoprecipitation.For complete details on the use and execution of this protocol, please refer to Daou et al. (2011).
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Immunoprecipitation and Western blot-based detection of protein O-GlcNAcylation in cells
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Autor/in / Beteiligte Person: | Ahmed, Oumaima ; Affar, Malik ; Masclef, Louis ; Echbicheb, Mohamed ; Gushul-Leclaire, Mila ; Estavoyer, Benjamin ; Vocadlo, David J. ; El Bachir Affar |
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Zeitschrift: | STAR Protocols, Jg. 3 (2022), Heft 1, S. 101108- |
Veröffentlichung: | Elsevier, 2022 |
Medientyp: | academicJournal |
ISSN: | 2666-1667 (print) |
DOI: | 10.1016/j.xpro.2021.101108 |
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