A protective multiple gene-deleted African swine fever virus genotype II, Georgia 2007/1, expressing a modified non-haemadsorbing CD2v protein ...
Taylor & Francis, 2023
Zeitungsartikel
Zugriff:
African swine fever virus is a complex DNA virus that causes high fatality in pigs and wild boar and has a great socio-economic impact. An attenuated genotype II strain was constructed by replacing the gene for wildtype CD2v protein with versions in which single or double amino acid substitutions were introduced to reduce or abrogate the binding to red blood cells and reduce virus persistence in blood. The mutant CD2v proteins were expressed at similar levels to the wildtype protein on the surface of infected cells. Three recombinant viruses also had K145R, EP153R, and in one virus DP148R genes deleted. Following immunization of pigs, the virus with a single amino acid substitution in CD2v, Q96R, induced moderate levels of replication, and 100% protection against virulent ASFV. Two additional recombinant viruses had two amino acid substitutions in CD2v, Q96R, and K108D, and induced no binding to red blood cells in vitro . In immunized pigs, reduced levels of virus in blood and strong early ASFV-specific ...
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A protective multiple gene-deleted African swine fever virus genotype II, Georgia 2007/1, expressing a modified non-haemadsorbing CD2v protein ...
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Autor/in / Beteiligte Person: | Rathakrishnan, Anusyah ; Reis, Ana L. ; Petrovan, Vlad ; Goatley, Lynnette C. ; Moffat, Katy ; Lui, Yuan ; Vuong, Mai T. ; Ikemizu, Shinji ; Davis, Simon J. ; Dixon, Linda K. |
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Veröffentlichung: | Taylor & Francis, 2023 |
Medientyp: | Zeitungsartikel |
DOI: | 10.6084/m9.figshare.24230523 |
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