Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli
In: Journal of Bacteriology, Jg. 134 (1978), Heft 1, S. 147-156
academicJournal
Zugriff:
Thiomethyl-beta-galactoside (TMG) accumulation via the melibiose transport system was studied in lactose transport-negative strains of Escherichia coli. TMG uptake by either intact cells or membrane vesicles was markedly stimulated by Na+ or Li+ between pH 5.5 and 8. The Km for uptake of TMG was approximately 0.2 mM at an external Na+ concentration of 5 mM (pH 7). The alpha-galactosides, melibiose, methyl-alpha-galactoside, and o-nitrophenyl-alpha-galactoside had a high affinity for this system whereas lactose, maltose and glucose had none. Evidence is presented for Li+-TMG or Na+-TMG cotransport.
| Titel: |
Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli
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| Autor/in / Beteiligte Person: | Lopilato, J ; Tsuchiya, T ; Wilson, T H |
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| Zeitschrift: | Journal of Bacteriology, Jg. 134 (1978), Heft 1, S. 147-156 |
| Veröffentlichung: | American Society for Microbiology, 1978 |
| Medientyp: | academicJournal |
| ISSN: | 0021-9193 |
| DOI: | 10.1128/jb.134.1.147-156.1978 |
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