Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya
In: Parasites & Vectors, Jg. 10 (2017), Heft 1, S. 1-13
Online
academicJournal
Zugriff:
Background Glossina pallidipes is a major vector of both Human and Animal African Trypanosomiasis (HAT and AAT) in Kenya. The disease imposes economic burden on endemic regions in Kenya, including south-western Kenya, which has undergone intense but unsuccessful tsetse fly control measures. We genotyped 387 G. pallidipes flies at 13 microsatellite markers to evaluate levels of temporal genetic variation in two regions that have been subjected to intensive eradication campaigns from the 1960s to the 1980s. One of the regions, Nguruman Escarpment, has been subject to habitat alteration due to human activities, while the other, Ruma National Park, has not. In addition, Nguruman Escarpment is impacted by the movement of grazing animals into the area from neighboring regions during the drought season. We collected our samples from three geographically close sampling sites for each of the two regions. Samples were collected between the years 2003 and 2015, spanning ~96 tsetse fly generations. Results We established that allelic richness averaged 3.49 and 3.63, and temporal Ne estimates averaged 594 in Nguruman Escarpment and 1120 in Ruma National Park. This suggests that genetic diversity is similar to what was found in previous studies of G. pallidipes in Uganda and Kenya, implying that we could not detect a reduction in genetic diversity following the extensive control efforts during the 1960s to the 1980s. However, we did find differences in temporal patterns of genetic variation between the two regions, indicated by clustering analysis, pairwise FST, and Fisher’s exact tests for changes in allele and genotype frequencies. In Nguruman Escarpment, findings indicated differentiation among samples collected in different years, and evidence of local genetic bottlenecks in two locations previous to 2003, and between 2009 and 2015. In contrast, there was no consistent evidence of differentiation among samples collected in different years, and no evidence of local genetic bottlenecks in Ruma National Park. ...
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Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya
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Autor/in / Beteiligte Person: | Okeyo, Winnie A. ; Saarman, Norah P. ; Mengual, Michael ; Dion, Kirstin ; Bateta, Rosemary ; Mireji, Paul O. ; Okoth, Sylvance ; Ouma, Johnson O. ; Ouma, Collins ; Ochieng, Joel ; Murilla, Grace ; Aksoy, Serap ; Caccone, Adalgisa |
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Zeitschrift: | Parasites & Vectors, Jg. 10 (2017), Heft 1, S. 1-13 |
Veröffentlichung: | BMC, 2017 |
Medientyp: | academicJournal |
ISSN: | 1756-3305 (print) |
DOI: | 10.1186/s13071-017-2415-y |
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