Using a miniaturized double-net trap (DN-Mini) to assess relationships between indoor–outdoor biting preferences and physiological ages of two malaria vectors, Anopheles arabiensis and Anopheles funestus
In: Malaria Journal, Jg. 18 (2019), Heft 1, S. 1-15
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Zugriff:
Background Effective malaria surveillance requires detailed assessments of mosquitoes biting indoors, where interventions such as insecticide-treated nets work best, and outdoors, where other interventions may be required. Such assessments often involve volunteers exposing their legs to attract mosquitoes [i.e., human landing catches (HLC)], a procedure with significant safety and ethical concerns. Here, an exposure-free, miniaturized, double-net trap (DN-Mini) is used to assess relationships between indoor–outdoor biting preferences of malaria vectors, Anopheles arabiensis and Anopheles funestus, and their physiological ages (approximated by parity and insemination states). Methods The DN-Mini is made of UV-resistant netting on a wooden frame and PVC base. At 100 cm × 60 cm × 180 cm, it fits indoors and outdoors. It has a protective inner chamber where a volunteer sits and collects host-seeking mosquitoes entrapped in an outer chamber. Experiments were conducted in eight Tanzanian villages using DN-Mini to: (a) estimate nightly biting and hourly biting proportions of mosquitoes indoors and outdoors; (b) compare these proportions to previous estimates by HLC in same villages; and, (c) compare distribution of parous (proxy for potentially infectious) and inseminated mosquitoes indoors and outdoors. Results More than twice as many An. arabiensis were caught outdoors as indoors (p < 0.001), while An. funestus catches were marginally higher indoors than outdoors (p = 0.201). Anopheles arabiensis caught outdoors also had higher parity and insemination proportions than those indoors (p < 0.001), while An. funestus indoors had higher parity and insemination than those outdoors (p = 0.04). Observations of indoor-biting and outdoor-biting proportions, hourly biting patterns and overall species diversities as measured by DN-Mini, matched previous HLC estimates. Conclusions Malaria vectors that are behaviourally adapted to bite humans outdoors also have their older, potentially infectious sub-populations .
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Using a miniaturized double-net trap (DN-Mini) to assess relationships between indoor–outdoor biting preferences and physiological ages of two malaria vectors, Anopheles arabiensis and Anopheles funestus
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Autor/in / Beteiligte Person: | Limwagu, Alex J. ; Kaindoa, Emmanuel W. ; Ngowo, Halfan S. ; Hape, Emmanuel ; Finda, Marceline ; Mkandawile, Gustav ; Kihonda, Japhet ; Kifungo, Khamis ; Njalambaha, Rukiyah M. ; Matoke-Muhia, Damaris ; Okumu, Fredros O. |
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Zeitschrift: | Malaria Journal, Jg. 18 (2019), Heft 1, S. 1-15 |
Veröffentlichung: | BMC ; BioMed Central, 2019 |
Medientyp: | academicJournal |
DOI: | 10.1186/s12936-019-2913-9 |
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