Localization and characterization of pituitary adenylate cyclase-activating polypeptide receptors in the human cerebellum during development
In: ISSN: 0021-9967, 2006
academicJournal
Zugriff:
International audience ; Pituitary adenylate cyclase-activating polypeptide (PACAP) receptors are actively expressed in the cortical layers of the cerebellum of rodents and contribute to cerebellar development. The present report provides the first anatomical localization and characterization of PACAP receptors in the developing human cerebellum. RT-PCR analysis from 15-week-old fetuses to 22-year-old subject showed that PAC1-R and VPAC1-R are expressed in the cerebellum at all stages, whereas VPAC2-R mRNA was barely detectable. In situ hybridization labeling indicated that, in human fetuses, PAC1-R mRNA is associated with the external granule cell layer (EGL), a germinative neuroepithelium, and with the internal granule cell layer (IGL). The distribution pattern of VPAC1-R mRNA was very similar to that of PAC1-R mRNA, whereas VPAC2-R mRNA was visualized only in 7-22-year-old subjects. The localization of [(125)I]PACAP27 binding sites was fully consistent with the distribution of PAC1-R and VPAC1-R mRNA. Pharmacological characterization revealed that, in the EGL and IGL from 15-24-week-old fetuses and in the granule cell layer from 7-22-year-old patients, binding sites exhibit a PAC1-R profile. In contrast, PACAP binding sites observed in the molecular layer and medulla of the adult cerebellum consisted of a heterogeneous population of PAC1-R and VPAC(1/2)-R. Altogether, these data provide the first evidence that PACAP receptors are expressed in the human cerebellar cortex. PAC1-R is the predominant PACAP receptor found in fetuses, and both PAC1-R and VPAC1-R are expressed in the mature cerebellum. These observations suggest that PACAP has neurodevelopmental functions in the human cerebellum.
Titel: |
Localization and characterization of pituitary adenylate cyclase-activating polypeptide receptors in the human cerebellum during development
|
---|---|
Autor/in / Beteiligte Person: | Basille, Magali ; Cartier, Dorthe ; Vaudry, David ; Lihrmann, Isabelle ; Fournier, Alain ; Fréger, Pierre ; Gallo-Payet, Nicole ; Vaudry, Hubert ; Gonzalez, Bruno ; Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (IFRMP 23) ; Rouen, CHU ; Normandie Université (NU)-Normandie Université (NU)-Université Le Havre Normandie (ULH) ; Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN) ; Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Lutte Contre le Cancer Henri Becquerel Normandie Rouen (CLCC Henri Becquerel)-Centre National de la Recherche Scientifique (CNRS) ; Neuroendocrinologie cellulaire et moléculaire ; Université de Rouen Normandie (UNIROUEN) ; Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM) ; Institut Armand Frappier (INRS-IAF) ; Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP) ; Service de Neurochirurgie CHU Rouen ; Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN) ; Normandie Université (NU) ; Université de Sherbrooke (UdeS) ; Institut National de la Santé et de la Recherche Médicale (INSERM). Grant Number: U‐413- INSERM‐ Fonds de la recherche en santé du Québec Exchange Program- Conseil Régional de Haute‐Normandie ; The authors thank Mr. Patrice Bizet, Mr. Gérard Cauchois, and Mrs. Huguette Lemonnier for excellent technical assistance. |
Link: | |
Zeitschrift: | ISSN: 0021-9967, 2006 |
Veröffentlichung: | HAL CCSD ; Wiley, 2006 |
Medientyp: | academicJournal |
DOI: | 10.1002/cne.20934 |
Schlagwort: |
|
Sonstiges: |
|