House dust mite-induced endoplasmic reticulum stress mediates MUC5AC hypersecretion via TBK1 in airway epithelium ...
Taylor & Francis, 2023
academicJournal
Zugriff:
Purpose: Endoplasmic reticulum (ER) stress regulates mucus hypersecretion, and may activate downstream factors via TBK1 signaling to induce gene expression. However, it remains unclear whether ER stress promotes airway mucus secretion through the TBK1 pathway. We aimed to investigate the role of the TBK1 pathway in the regulation of MUC5AC expression in a mouse model of house dust mite (HDM)-induced allergic asthma. Materials and Methods: Mice with HDM-induced asthma and human bronchial epithelial BEAS-2B cells were treated with amlexanox, an anti-allergy drug (25 μM), or 4-PBA (10 mM). Tissue and cell samples were collected. Tissue samples were stained with hematoxylin and eosin (H&E) or periodic acid Schiff (PAS) to evaluate pathology. Protein expression was analyzed by western blotting and immunofluorescence. Results: Mice exposed to HDM presented ER stress and hypersecretion of mucus Muc5ac from airway epithelial cells ( p < 0.001). Similar results were observed in BEAS-2B cells following exposure ...
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House dust mite-induced endoplasmic reticulum stress mediates MUC5AC hypersecretion via TBK1 in airway epithelium ...
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Autor/in / Beteiligte Person: | Deng, Jun ; Tang, Hongmei ; Zhang, Yun ; Yuan, Xiefang ; Ma, Ning ; Hu, Hang ; Wang, Xiaoyun ; Liu, Chunfeng ; Xu, Guofeng ; Li, Yuejiao ; Wang, Songping ; Guo, Linlin ; Wang, Xing |
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Veröffentlichung: | Taylor & Francis, 2023 |
Medientyp: | academicJournal |
DOI: | 10.6084/m9.figshare.21982427 |
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