Genomic variants are selected in ulp2Δ/Δ cells challenged with Fluconazole.

(A) Whole genome sequence data were plotted as the log2 ratio and converted to chromosome copy number (y-axis, 1–4 copies) as a function of chromosome position (x-axis, Chr1-ChrR) using YMAP. Heterozygous (AB) regions are indicated with gray shading and homozygous regions are indicated by haplotype AA (cyan) or BB (magenta). Allele ratio changes that occur within a CNV are indicated as dark blue (AAB) or purple (ABB). Colony B and C had allele ratio colouring that was corrected using IGV and allele frequency information. (B) Serial dilution assay of ulp2Δ/ Δ parental (P) and fluconazole-recovered isolates ( FLC-2 , FLC-3 , FLC-4 , FLC-1a , FLC-1b and FLC-1c ) in non-selective (N/S) or media containing 128 μg/ml fluconazole (FLC). (PDF)