Characterization of tbc-11(qbc24) and tbc-11(ok2576) mutant alleles.
2021
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(A) GFP reporter repressed by λN::mCherry tagged ALG-1. GFP was fused to alg-1 endogenous promoter ( alg-1p ) and cog-1 3’ UTR in which the lsy-6 binding sites were replaced by 6 box B sequences (cog-1Δlsy-6 bs). ( B ) DIC and fluorescent microscopy of WT and tbc-11(qbc24) pharynx in young adults. GFP is derepressed in tbc-11(qbc24) animals. ALG-1 expression (represented by mCherry) is not affected in these animals. Images were taken using the same settings and exposition time for each animal. Scale bar: 50μm. ( C ) Percentage of alae breaks of wild-type (WT), tbc-11(qbc24) homozygous, and tbc-11(qbc24) heterozygous ( tbc-11(qbc24 )/+) young adult animals. tbc-11(qbc24) heterozygous animals were observed as F1 of a genetic cross between tbc-11(qbc24) and wild-type animals. P value were obtained by one-way ANOVA (**p value < 0.001) ( D ) Number of seam cells in tbc-11(qbc24) and (ok2576) alleles. Strains were crossed with a strain expressing GFP in the seam cells ( scm ::GFP) in order to score them. Animals were observed as young adults. Wild-type (WT) animals have an invariable number of 16 seam cells. P value were obtained by Fisher’s exact test (***p value < 0.0001) ( E ) Left: DIC and fluorescent microscopy of col-10 :: gfp :: lin-41 3’UTR reporter in hypodermal cells of L4 staged animals. Animals were fed with bacteria expressing RNAi against tbc-11 or control RNAi (no targeting gene) for 48 hours. Scale bar: 50μm. Right: Quantification of GFP fluorescence intensity in four hypodermal cells per animal. Images were taken using the same settings and exposition time for each animal. The number of animals scored (n) is indicated. P value were obtained by two tailed t-test. (* p value < 0.05). (F) Left: DIC and fluorescent microscopy of miR-228 mutated reporter in intestine cells of L2 staged animals. Scale bar: 50μm. Right: Quantification of GFP fluorescence intensity in four intestine cells per animal. Images were taken using the same settings and exposition time for each animal. The number of animals ...
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Characterization of tbc-11(qbc24) and tbc-11(ok2576) mutant alleles.
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Autor/in / Beteiligte Person: | Pascale Michaud (3389318) ; Vivek Nilesh Shah (10535868) ; Pauline Adjibade (8796008) ; Francois Houle (10535871) ; Miguel Quévillon Huberdeau (3389315) ; Rachel Rioux (10535874) ; Camille Lavoie-Ouellet (10535877) ; Weifeng Gu (563224) ; Rachid Mazroui (219768) ; Martin J. Simard (10535880) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pgen.1009511.s001 |
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