Adaptive differentiation of Plasmodium falciparum populations inferred from single-nucleotide polymorphisms (SNPs) conferring drug resistance and from neutral SNPs.
In: ISSN: 0022-1899, 2010
Online
academicJournal
Zugriff:
International audience ; BACKGROUND: Theoretical and experimental data support the geographic differentiation strategy as a valuable tool for detecting loci under selection. In the context of Plasmodium falciparum malaria, few populations have been studied, with limited genomic coverage. METHODS: We examined geographic differentiation in P. falciparum populations on the basis of 12 single-nucleotide polymorphisms (SNPs) in 4 genes encoding drug resistance determinants, 5 SNPs in 2 genes encoding antigens, and a set of 17 putatively neutral SNPs dispersed on 13 chromosomes. We sampled 326 parasite isolates representing 7 P. falciparum populations from regions with varied levels of malaria transmission (Gabon, Kenya, Madagascar, Mali, Mayotte, Haiti, and the Philippines). RESULTS: Frequencies of drug resistance alleles varied considerably among populations (mean F(ST), 0.52). In contrast, allele frequencies varied significantly less for antigenic and neutral SNPs (mean F(ST), 0.16 and 0.24, respectively). This contrasting pattern was more pronounced when only the African populations were considered. Signature of selection was detected for most of the resistant SNPs but not for the antigenic SNPs. CONCLUSION: These data further validate the utility of geographic differentiation for identifying loci under strong positive selection, such as drug resistance loci. This study also provides frequencies of molecular makers of resistance in some overlooked populations.
Titel: |
Adaptive differentiation of Plasmodium falciparum populations inferred from single-nucleotide polymorphisms (SNPs) conferring drug resistance and from neutral SNPs.
|
---|---|
Autor/in / Beteiligte Person: | Maïga-Ascofaré, Oumou ; Le Bras, Jacques ; Mazmouz, Rabia ; Renard, Emmanuelle ; Falcão, Sarah ; Broussier, Emeline ; Bustos, Dorina ; Randrianarivelojosia, Milijaona ; Omar, Sabah A ; Aubouy, Agnès ; Lepère, Jean-François ; Jean-François, Vély ; Djimdé, Abdoulaye A ; Clain, Jérôme ; Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 216) ; Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5) ; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Medicine, Pharmacy, and Odontostomatology ; Université de Bamako ; Centre de consultations de Bandraboua ; Research Institute for Tropical Medicine ; Institut Pasteur de Madagascar ; Réseau International des Instituts Pasteur (RIIP) ; Kenya Medical Research Institute (KEMRI) ; Unité de Recherche 010 ; IRD ; Service National de Lutte Antipaludique ; Financial support: French Ministry of Health (grant to the Centre National de Réference du Paludisme) ; Institut de Médecine et d'Epidémiologie Appliquée/Fondation Léon Mba (grant 5988CLN90PFGO) ; French Ministry of Foreign Affairs for the France-Philippine Technical Cooperation Project on Malaria (grant) ; Bio-MalPar Network of Excellence. |
Link: | |
Zeitschrift: | ISSN: 0022-1899, 2010 |
Veröffentlichung: | HAL CCSD ; Oxford University Press, 2010 |
Medientyp: | academicJournal |
DOI: | 10.1086/656142 |
Schlagwort: |
|
Sonstiges: |
|