Comparison of Plasmid-Mediated Quinolone Resistance in Escherichia coli Isolates from Livestock and Food in Germany

drugs. Resistance to quinolones can be chromosomally encoded or plasmid-mediated (PMQR). One PMQR mechanism is mediated by Qnr proteins. The horizontal gene transfer of this plasmid-mediated quinolone resistance increases the threat of fallible treatment with some antibiotics. To better understand the qnr PMQR pathway as well as the distribution of qnr genes, Escherichia (E.) coli isolates recovered in 2016 and 2017 from livestock and food were phenotypically and genotypically characterized. Materials/methods: 6,817 E. coli isolates from the German National Reference Laboratory for Antimicrobial Resistance were investigated. The isolates were received in the German national monitoring program for antimicrobial resistance in zoonotic bacteria, recovered from livestock and food. Antimicrobial resistance was determined by broth microdilution according to CLSI guidelines. MIC values were evaluated using EUCAST epidemiological cut-off values. E. coli resistant to quinolones were subjected to qnr -PCR, XbaI-PFGE, S1-PFGE, WGS and bioinformatic analysis. Six different qnr -PCRs were conducted to identify the respective qnr -variants. Results: Of 6,817 E. coli tested, more than 800 isolates were classified as quinolone-resistant (MIC NAL ≥16 mg/L and/or MIC CIP ≥0.06 mg/L). The most abundant qnr -variant was qnrS . With the exception of qnrD, other qnr -variants were found evenly distributed. In general, E. coli isolates with qnr -genes were found more frequently in the feces of animals than in the meat of the same animal species. PFGE with XbaI-digestion was performed to examine genetic relatedness of isolates. PFGE profiling demonstrated a rather high heterogeneity. The highly diverse PFGE pattern indicates that the screened isolates are not associated to a predominant E. coli clone spreading via vertical transmission. S1-PFGE plasmid profiling showed a variety of extrachromosomal elements of various sizes. Isolates, selected according their PFGE pattern were further screened for their genetic setting through short ...