AMP-activated protein kinase and metabolic regulation in cold-hardy insects.
In: Journal of Insect Physiology, Vol. 57, no.11, p. 1453-62 (2011, 2011
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Zugriff:
Winter survival for many insects depends on cold hardiness adaptations as well as entry into a hypometabolic diapause state that minimizes energy expenditure. We investigated whether AMP-activated protein kinase (AMPK) could be involved in this adaptation in larvae of two cold-hardy insects, Eurosta solidaginis that is freeze tolerant and Epiblema scudderiana that uses a freeze avoidance strategy. AMPK activity was almost 2-fold higher in winter larvae (February) compared with animals collected in September. Immunoblotting revealed that phosphorylation of AMPK in the activation loop and phosphorylation of acetyl-CoA carboxylase (ACC), a key target of AMPK, were higher in Epiblema during midwinter whereas no seasonal change was seen in Eurosta. Immunoblotting also revealed a significant increase in ribosomal protein S6 phosphorylation in overwintering Epiblema larvae, and in both Eurosta and Epiblema, phosphorylation of eukaryotic initiation factor 4E-binding protein-1 dramatically increased in the winter. Pyruvate dehydrogenase (PDH) E1α subunit site 1 phosphorylation was 2-fold higher in extracts of Eurosta larvae collected in February versus September while PDH activity decreased by about 50% in Eurosta and 80% in February Eurosta larvae compared with animals collected in September. Glycogen phosphorylase phosphorylation was 3-fold higher in Epiblema larvae collected in February compared with September and also in these animals, triglyceride lipase activity increased by 70% during winter. Overall, our study suggests a re-sculpting of metabolism during insect diapause, which shifted to a more catabolic poise in freeze-avoiding overwintering Epiblema larvae, possibly involving AMPK.
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AMP-activated protein kinase and metabolic regulation in cold-hardy insects.
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Autor/in / Beteiligte Person: | Rider, Mark ; Hussain, Nusrat ; Dilworth, Stephen M ; Storey, Janet M ; Storey, Kenneth B ; UCL - SSS/DDUV - Institut de Duve |
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Zeitschrift: | Journal of Insect Physiology, Vol. 57, no.11, p. 1453-62 (2011, 2011 |
Veröffentlichung: | Pergamon, 2011 |
Medientyp: | academicJournal |
ISSN: | 0022-1910 (print) ; 1879-1611 (print) |
DOI: | 10.1016/j.jinsphys.2011.07.006 |
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