Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA
In: ISSN: 2075-4418 ; Diagnostics ; https://hal.univ-reunion.fr/hal-04514302 ; Diagnostics, 2023, 13 (3), ⟨10.3390/diagnostics13030462⟩, 2023
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Zugriff:
International audience ; Zika virus (ZIKV) diagnostics are crucial for proper antenatal and postnatal care and also for surveillance and serosurvey studies. Since the viremia during ZIKV infection is fleeting, serological testing is highly valuable to inform diagnosis. However, current serology tests using whole virus antigens frequently suffer from cross reactivity issues, delays, and technical complexity, especially in low and middle income countries (LMICs) and endemic countries. Here, we describe an indirect ELISA to detect specific IgG antibodies using the ZIKV envelope domain III (EDIII) protein expressed in Drosophila S2 cells as an immunogen. Using a total of 367 clinical samples, we showed that the EDIII-ELISA was able to detect IgG antibodies against ZIKV with high sensitivity of 100.0% and specificity of 94.7% when compared to plaque reduction neutralization tests (PRNTs) as the gold standard and using 0.208 as the cut-off OD value. These results show the usefulness of the recombinant envelope domain III as an alternative to standard whole virus proteins for ZIKV diagnostics as it improves the sensitivity and specificity of IgG ELISA assay when used as an immunogen. This method should, therefore, be extended to serological diagnostic techniques for other members of the flavivirus genus and for use in IgM diagnostic testing.
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Use of Envelope Domain III Protein for the Detection of IgG Type Antibodies Specific to Zika Virus by Indirect ELISA
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Autor/in / Beteiligte Person: | Ndiaye, Oumar ; Diagne, Cheikh, Tidiane ; Abd El Wahed, Ahmed ; Dia, Fatou ; Dia, Moussa ; Faye, Adama ; Leal, Silvania, da Veiga ; dos Santos, Menilita ; Lima Mendonça, Maria, da Luz De ; da Silva Leite, Carolina, Cardoso ; Bouh Boye, Cheikh, Saad ; Bryant, Juliet, E ; Desprès, Philippe ; Faye, Ousmane ; Sall, Amadou, Alpha ; Faye, Oumar ; Institut Pasteur de Dakar ; Réseau International des Instituts Pasteur (RIIP) ; Université Cheikh Anta Diop de Dakar Sénégal (UCAD) ; Leipzig University / Universität Leipzig ; Ministry of Health Cape Verde ; Aristide-Le-Dantec, Hôpital ; Laboratoire des pathogènes émergents -- Emerging Pathogens Laboratory (LPE-Fondation Mérieux) ; Centre International de Recherche en Infectiologie (CIRI) ; École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) ; Processus Infectieux en Milieu Insulaire Tropical (PIMIT) ; Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS) ; Département de virologie - Virology Department Dakar ; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) |
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Zeitschrift: | ISSN: 2075-4418 ; Diagnostics ; https://hal.univ-reunion.fr/hal-04514302 ; Diagnostics, 2023, 13 (3), ⟨10.3390/diagnostics13030462⟩, 2023 |
Veröffentlichung: | HAL CCSD ; MDPI, 2023 |
Medientyp: | academicJournal |
DOI: | 10.3390/diagnostics13030462 |
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