Impaired Functional T-Cell Response to SARS-CoV-2 After Two Doses of BNT162b2 mRNA Vaccine in Older People
In: Frontiers in Immunology ; volume 12 ; ISSN 1664-3224, 2021
academicJournal
Zugriff:
Long-term care facility (LTCF) older residents display physiological alterations of cellular and humoral immunity that affect vaccine responses. Preliminary reports suggested a low early postvaccination antibody response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aim of this study was to focus on the specific T-cell response. We quantified S1-specific IgG, neutralizing antibody titers, total specific IFNγ-secreting T cells by ELISpot, and functionality of CD4 + - and CD8 + -specific T cells by flow cytometry, after two doses of the BNT162b2 vaccine in younger and older people, with and without previous COVID-19 infection (hereafter referred to as COVID-19-recovered and COVID-19-naive subjects, respectively). Frailty, nutritional, and immunosenescence parameters were collected at baseline in COVID-19-naive older people. We analyzed the immune response in 129 young adults (median age 44.0 years) and 105 older residents living in a LCTF (median age 86.5 years), 3 months after the first injection. Humoral and cellular memory responses were dramatically impaired in the COVID-19-naive older ( n = 54) compared with the COVID-19-naive younger adults ( n = 121). Notably, older participants’ neutralizing antibodies were 10 times lower than the younger’s antibody titers ( p < 0.0001) and LCTF residents also had an impaired functional T-cell response: the frequencies of IFNγ + and IFNγ + IL-2 + TNFα + cells among specific CD4 + T cells, and the frequency of specific CD8 + T cells were lower in COVID-19-naive older participants than in COVID-19-naive young adults ( p < 0.0001 and p = 0.0018, respectively). However, COVID-19-recovered older participants ( n = 51) had greater antibody and T-cell responses, including IFNγ + and IFNγ + IL-2 + TNFα + -specific CD4 + T cells ( p < 0.0001), as well as TNFα + -specific CD8 + T cells ( p < 0.001), than COVID-19-naive older adults. We also observed that “inflammageing” and particularly high plasma levels of TNFα was associated ...
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Impaired Functional T-Cell Response to SARS-CoV-2 After Two Doses of BNT162b2 mRNA Vaccine in Older People
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Autor/in / Beteiligte Person: | Demaret, Julie ; Corroyer-Simovic, Bénédicte ; Alidjinou, Enagnon Kazali ; Goffard, Anne ; Trauet, Jacques ; Miczek, Sophie ; Vuotto, Fanny ; Dendooven, Arnaud ; Huvent-Grelle, Dominique ; Podvin, Juliette ; Dreuil, Daniel ; Faure, Karine ; Deplanque, Dominique ; Bocket, Laurence ; Duhamel, Alain ; Labreuche, Julien ; Sobaszek, Annie ; Hisbergues, Michael ; Puisieux, Francois ; Labalette, Myriam ; Lefèvre, Guillaume |
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Zeitschrift: | Frontiers in Immunology ; volume 12 ; ISSN 1664-3224, 2021 |
Veröffentlichung: | Frontiers Media SA, 2021 |
Medientyp: | academicJournal |
DOI: | 10.3389/fimmu.2021.778679 |
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