Single‐turnover flash‐induced ATP synthesis in photosystem I‐enriched subchloroplast vesicles
In: FEBS Letters, Jg. 192 (1985), Heft 2, S. 271-274
academicJournal
Zugriff:
Flash‐induced ATP synthesis coupled to cyclic electron flow in photosystem I‐enriched subchloroplast vesicles was studied by continuous registration of luciferin‐luciferase luminescence. Cyclic electron flow was elicited in the presence of the artificial mediator phenazine methosulphate or of native ferredoxin, the latter redox‐poised by NADPH and oxygen. ATP hydrolysis is observed in dark‐adapted vesicles in the presence of ferredoxin without requirement for previous activation of the ATPases. Ferredoxin‐catalyzed ATP synthesis proceeds at a constant (maximal) rate between NADPH/O 2 ; ratios of 1.5 and 11. It was sensitive to FCCP, DCCD, DBMIB, DNP‐INT and HQNO, but not to antimycin A.
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Single‐turnover flash‐induced ATP synthesis in photosystem I‐enriched subchloroplast vesicles
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Autor/in / Beteiligte Person: | De Wolf, F.A. ; Galmiche, J.M. ; Kraayenhof, R. ; Girault, G. |
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Zeitschrift: | FEBS Letters, Jg. 192 (1985), Heft 2, S. 271-274 |
Veröffentlichung: | Wiley, 1985 |
Medientyp: | academicJournal |
ISSN: | 0014-5793 |
DOI: | 10.1016/0014-5793(85)80123-x |
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