tbc-11 affects ALG-1 intracellular localization.

(A) Fluorescent microscopy of intracellular localization of endogenously tagged GFP::ALG-1 in seam cells of wild-type (WT) and tbc-11(qbc24) young adults animals. Animals were fed with bacteria expressing RNAi against rab-6 . 1 or control RNAi (no targeting gene) for 48 hours. The nucleus of the seam cell is indicated by a yellow arrow. A zoomed in image of tbc-11(qbc24) seam cell is showed. Images were taken using the same settings and exposition time for each animal. Scale bar: 10μm. ( B ) Quantification of representative images of GFP::ALG-1 in seam cells of wild-type (WT) and tbc-11(qbc24) animals exposed to RNAi against rab-6 . 1 or control RNAi (no targeting gene) for 48 hours. Quantification was performed by drawing a straight line starting from the middle of the nucleus and passing through the cytoplasm of the seam cell. The fluorescence signal was measured along the line using ImageJ. The distance in μm represents the distance from the middle of the nucleus. Each line represents a separate image of a different animal.