Gastrin-Releasing Peptide Promotes Suprachiasmatic Nuclei Cellular Rhythmicity in the Absence of Vasoactive Intestinal Polypeptide-VPAC(2) Receptor Signaling
Society for Neuroscience, 2005
academicJournal
Zugriff:
Vasoactive intestinal polypeptide (VIP) and gastrin-releasing peptide (GRP) acting via the VPAC(2) receptor and BB(2) receptors, respectively, are key signaling pathways in the suprachiasmatic nuclei (SCN) circadian clock. Transgenic mice lacking the VPAC(2) receptor (Vipr2(-/-)) display a continuum of disrupted behavioral rhythms with only a minority capable of sustaining predictable cycles of rest and activity. However, electrical or molecular oscillations have not yet been detected in SCN cells from adult Vipr2(-/-) mice. Using a novel electrophysiological recording technique, we found that in brain slices from wild-type and behaviorally rhythmic Vipr2(-/-) mice, the majority of SCN neurons we detected displayed circadian firing patterns with estimated periods similar to the animals' behavior. In contrast, in slices from behaviorally arrhythmic Vipr2(-/-) mice, only a small minority of the observed SCN cells oscillated. Remarkably, exogenous GRP promoted SCN cellular rhythms in Vipr2(-/-) mouse slices, whereas blockade of BB(2) receptors suppressed neuronal oscillations. In wild-type mice, perturbation of GRP-BB(2) signaling had few effects on SCN cellular rhythms, except when VPAC(2) receptors were blocked pharmacologically. These findings establish that residual electrical oscillations persist in the SCN of Vipr2(-/-) mice and reveal a potential new role for GRP-BB(2) signaling within the circadian clock.
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Gastrin-Releasing Peptide Promotes Suprachiasmatic Nuclei Cellular Rhythmicity in the Absence of Vasoactive Intestinal Polypeptide-VPAC(2) Receptor Signaling
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Autor/in / Beteiligte Person: | Brown, Timothy M. ; Hughes, Alun T. ; Piggins, Hugh D. |
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Veröffentlichung: | Society for Neuroscience, 2005 |
Medientyp: | academicJournal |
DOI: | 10.1523/JNEUROSCI.3821-05.2005 |
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