Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology Tools and Expansion of the Ligandable Proteome
In: ACS Chem Biol, 2023
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Zugriff:
[Image: see text] Here, we report a comprehensive profiling of sulfur(VI) fluorides (S(VI)-Fs) as reactive groups for chemical biology applications. S(VI)-Fs are reactive functionalities that modify lysine, tyrosine, histidine, and serine sidechains. A panel of S(VI)-Fs were studied with respect to hydrolytic stability and reactivity with nucleophilic amino acid sidechains. The use of S(VI)-Fs to covalently modify carbonic anhydrase II (CAII) and a range of kinases was then investigated. Finally, the S(VI)-F panel was used in live cell chemoproteomic workflows, identifying novel protein targets based on the type of S(VI)-F used. This work highlights how S(VI)-F reactivity can be used as a tool to expand the liganded proteome.
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Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology Tools and Expansion of the Ligandable Proteome
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Autor/in / Beteiligte Person: | Gilbert, Katharine E. ; Vuorinen, Aini ; Aatkar, Arron ; Pogány, Peter ; Pettinger, Jonathan ; Grant, Emma K. ; Kirkpatrick, Joanna M. ; Rittinger, Katrin ; House, David ; Burley, Glenn A. ; Bush, Jacob T. |
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Zeitschrift: | ACS Chem Biol, 2023 |
Veröffentlichung: | American Chemical Society, 2023 |
Medientyp: | academicJournal |
DOI: | 10.1021/acschembio.2c00633 |
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