Diversity shift in bacterial phenol hydroxylases driven by alkyl-phenols in oil refinery wastewaters
In: Hyper Article en Ligne - Sciences de l'Homme et de la Société, 2017
academicJournal
Zugriff:
International audience Phenol hydroxylases (PHs) play a primary role in the bacterial degradation of phenol and alkylphenols. They are divided into two main classes, single-component and multi-component PHs, having distinctive catalytic subunits designated as PheA1 and LmPH, respectively. The diversity of these enzymes is still largely unexplored. Here, both LmPH and pheA1 gene sequences were examined in activated sludge from oil refinery wastewaters. Phenol, p-cresol, or 3,4-dimethylphenol (3,4-DMP) supplied as extra carbon sources were rapidly mineralized by the microbial community. Analysis of LmPH genes revealed a wide range of sequences, most of which exhibited moderate similarity with homologs found in Proteobacteria. Moreover, the LmPH diversity profiles showed a dramatic shift upon sludge treatment with p-cresol or 3,4-DMP amendment. This resulted in an enrichment in sequences similar to LmPHs from Betaproteobacteria and Gammaproteobacteria. RT-PCR analysis of RNA extracted from wastewater sludge highlighted LmPH genes best expressed in situ. A PCR approach was implemented to analyze the pheA1 gene diversity in the same microbial community. Retrieved sequences fell into four clusters and appeared to be distantly related to pheA1 genes from Actinobacteria. Altogether, our results provide evidence that phenol degraders carrying LmPH are more diverse than PheA1 carrying bacteria and suggest that PHs with best adapted substrate specificity are recruited in response to (methyl)phenol availability.
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Diversity shift in bacterial phenol hydroxylases driven by alkyl-phenols in oil refinery wastewaters
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Autor/in / Beteiligte Person: | Besma, Harzallah ; Bousseboua, Hacène ; Jouanneau, Yves ; Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249) ; Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) ; Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) ; Laboratoire de Génie Microbiologique et Applications ; FSNV, Université des Frères Mentouri, BP 325 25117, Constantine, Algeria. ; Département de Biologie ; FSESNVUniversité d’Oum El BouaghiOum El BouaghiAlgeria ; Ecole Nationale Supérieure de Biotechnologies ; Ecole Nationale Supérieure de BiotechnologiesConstantineAlgeria |
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Zeitschrift: | Hyper Article en Ligne - Sciences de l'Homme et de la Société, 2017 |
Veröffentlichung: | HAL CCSD ; Springer Verlag, 2017 |
Medientyp: | academicJournal |
DOI: | 10.1007/s11356-017-8950-4 |
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