Abyssinone V-4′ Methyl Ether, a Flavanone Isolated from Erythrina droogmansiana, Exhibits Cytotoxic Effects on Human Breast Cancer Cells by Induction of Apoptosis and Suppression of Invasion
In: Evidence-Based Complementary and Alternative Medicine, Vol 2020 (2020, 2020
Online
academicJournal
Zugriff:
Abyssinone V-4′ methyl ether (AVME) isolated from Erythrina droogmansiana was recently reported to exhibit anti-mammary tumor effect in mice. The present work was therefore aimed at elucidating its cellular and molecular mechanisms. To achieve our goal, the cytotoxicity of AVME against tumoral and non-tumoral cell lines was evaluated by resazurin reduction test; flow cytometry allowed us to evaluate the cell cycle and mechanisms of cell death; the mitochondrial transmembrane potential, reactive oxygen species (ROS) levels, and caspase activities as well as apoptosis-regulatory proteins (Bcl-2 and Bcl-XL) were measured in MDA-MB-231 cells. Further, the antimetastatic potential of AVME was evaluated by invasion assay. AVME exhibited cytotoxic effects in all tested tumor cell lines and induced a significant increase in the percentage of MDA-MB-231 cells at G2/M and S phases of the cell cycle in a concentration-dependent manner. AVME also induced apoptosis in MDA-MB-231 cells, which was accompanied by the activation of caspase-3 and caspase-9 and downregulation of Bcl-2 and Bcl-XL proteins. Moreover, AVME suppressed cancer cell invasion by the inhibition of the metalloproteinase-9 activity. Findings from this study suggest that AVME has anti-breast cancer activities expressed through mitochondrial proapoptotic pathway including impairment of aggressive behaviors of breast cancer cells.
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Abyssinone V-4′ Methyl Ether, a Flavanone Isolated from Erythrina droogmansiana, Exhibits Cytotoxic Effects on Human Breast Cancer Cells by Induction of Apoptosis and Suppression of Invasion
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Autor/in / Beteiligte Person: | Zingue, Stéphane ; Abel Joël Gbaweng Yaya ; Cisilotto, Julia ; Larissa Vanelle Kenmogne ; Talla, Emmanuel ; Bishayee, Anupam ; Njamen, Dieudonne ; Tânia Beatriz Creczynski-Pasa ; Derek Tantoh Ndinteh |
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Zeitschrift: | Evidence-Based Complementary and Alternative Medicine, Vol 2020 (2020, 2020 |
Veröffentlichung: | Hindawi Limited, 2020 |
Medientyp: | academicJournal |
ISSN: | 1741-427X (print) ; 1741-4288 (print) |
DOI: | 10.1155/2020/6454853 |
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