Phosphatidylinositol-dependent phospholipases C Plc2 and Plc3 of Candida albicans are dispensable for morphogenesis and host-pathogen interaction
In: ISSN: 0923-2508, 2005
academicJournal
Zugriff:
International audience ; Phospholipases play an important role as virulence factors in human pathogens. Candida albicans, the major fungal pathogen of humans, encodes phospholipases of type A, B, C and D. Type B Plb2 and type D Pld1 phospholipases have been shown to contribute to virulence in this organism. We analyzed, in C. albicans, PLC2 and PLC3, two highly conserved genes coding for phosphatidylinositol-dependent phospholipases C with homology to the known virulence factor PlcA in the human pathogen Listeria monocytogenes. We show that expression of PLC2 and PLC3 is upregulated under different filament-inducing conditions and in the constitutive filamentous mutant tup1Δ. In order to analyze PLC2 and PLC3 function in C. albicans, we constructed strains that carry PLC2 or PLC3 under a constitutive promoter and strains that lack all four PLC2/3 alleles. These strains were not affected in their ability to produce filaments under non-inducing conditions, nor was filamentation modified under inducing conditions, suggesting that PLC2/3 are not critical determinants of the yeast-to-hypha switch. In a cell culture model for macrophage interaction, phagocytosis of C. albicans and subsequent killing were not influenced by PLC2/3. These results demonstrate that C. albicans PLC2 and PLC3 are dispensable for virulence; moreover, they underline the sharp contrast with the function of plcA in L. monocytogenes.
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Phosphatidylinositol-dependent phospholipases C Plc2 and Plc3 of Candida albicans are dispensable for morphogenesis and host-pathogen interaction
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Autor/in / Beteiligte Person: | Knechtle, Philipp ; Goyard, Sophie ; Brachat, Sophie ; Ibrahim-Granet, Oumaïma ; d'Enfert, Christophe ; Biologie et Pathogénicité fongiques (BPF) ; Institut National de la Recherche Agronomique (INRA)-Institut Pasteur Paris (IP) ; Biozentrum ; Aspergillus ; Institut Pasteur Paris (IP) |
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Zeitschrift: | ISSN: 0923-2508, 2005 |
Veröffentlichung: | HAL CCSD ; Elsevier, 2005 |
Medientyp: | academicJournal |
DOI: | 10.1016/j.resmic.2005.04.007 |
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