Estandarización de un protocolo para la obtención de células diferenciables a multilinaje por medio de estrés químico

Giraldo Palacios, Alvaro José ; Neuta Arciniegas, Paola Andrea ; et al.

In: [1] Y. Kuroda et al., “Unique multipotent cells in adult human mesenchymal cell populations,” Proceedings of the National Academy of Sciences, vol. 107, no. 19, pp. 8639–8643, Mayo, 2010. doi:10.1073/pnas.0911647107.; [2] S. Heneidi et al., “Awakened by cellular tress: Isolation and characterization of a novel population of pluripotent stem cells derived from human adipose tissue,” PLOS ONE, vol. 8, no. 6, p. e64752, Jun., 2013. doi:10.1371/journal.pone.0064752; [3] Z. Yang et al., “Isolation and characterization of ssea3+ stem cells derived from goat skin fibroblasts,” Cellular Reprogramming, vol. 15, no. 3, pp. 195–205, Jun., 2013. doi:10.1089/cell.2012.0080.; [4] M. Iseki et al., “Human muse cells, nontumorigenic phiripotent-like stem cells, have liver regeneration capacity through specific homing and cell replacement in a mouse model of liver fibrosis,” Cell Transplantation, vol. 26, no. 5, pp. 821– 840, Mayo, 2017. doi:10.3727/096368916X693662.; [5] K. Kinoshita et al., “Therapeutic potential of adipose-derived ssea-3-positive muse cells for treating diabetic skin ulcers,” STEM CELLS Translational Medicine, vol. 4, no. 2, pp. 146–155, Ene., 2015. doi:10.5966/sctm.2014-0181.; [6] H. Uchida et al., “Transplantation of unique subpopulation of fibroblasts, muse cells, ameliorates experimental stroke possibly via robust neuronal differentiation,” STEM CELLS, vol. 34, no. 1, pp. 160–173, Ene., 2015. doi:10.1002/stem.2206.; [7] T. Yamauchi, K. Yamasaki, K. Tsuchiyama, S. Koike y S. Aiba, “The potential of muse cells for regenerative medicine of skin: Procedures to reconstitute skin with muse cell-derived keratinocytes, fibroblasts, and melanocytes,” Journal of Investigative Dermatology, vol. 137, no. 12, pp. 2639–2642, Dic., 2017. doi:10.1016/j.jid.2017.06.021.; [8] A. M. Fouad et al., “In vitro differentiation of human multilineage differentiating stress-enduring (Muse) cells into insulin producing cells,” Journal of Genetic Engineering and Biotechnology, vol. 16, no. 2, pp. 433–440, Dic., 2018. doi:10.1016/j.jgeb.2018.09.003.; [9] E. Toyoda et al., “Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue,” Regenerative Therapy, vol. 10, pp. 17–26, Jun., 2019. doi:10.1016/j.reth.2018.10.005.; [10] M. Dezawa, “Clinical Trials of Muse Cells” en Muse Cells. Japón: Springer Japan, 2018, cap. 17, p. 308.; [11] Y. Kuroda, S. Wakao, M. Kitada, T. Murakami, M. Nojima y M. Dezawa, “Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells,” Nature Protocols, vol. 8, no. 7, pp. 1391–1415, Jun., 2013. doi:10.1038/nprot.2013.076.; [12] K. Tatsumi, Y. Kushida, S. Wakao, Y. Kuroda y M. Dezawa, “Protocols for Isolation and Evaluation of Muse Cells,” en Advances in Experimental Medicine and Biology. Japón: Springer Japan, 2018, pp. 69–101. doi:10.1007/978-4- 431-56847-6_4.; [13] S. C. Fisch et al., “Pluripotent nontumorigenic multilineage differentiating stress enduring cells (Muse cells): A seven-year retrospective,” Stem Cell Research & Therapy, vol. 8, no. 1, Oct., 2017. doi:10.1186/s13287-017-0674-3.; [14] S. A. Przyborski, “Differentiation of human embryonic stem cells after transplantation in immune-deficient mice,” STEM CELLS, vol. 23, no. 9, pp. 1242–1250, Oct., 2005. doi:10.1634/stemcells.2005-0014.; [15] A. A. Simerman, D. A. Dumesic y G. D. Chazenbalk, “Pluripotent muse cells derived from human adipose tissue: A new perspective on regenerative medicine and cell therapy,” Clinical and Translational Medicine, vol. 3, no. 1, pp. 3-12, Mayo, 2014. doi:10.1186/2001-1326-3-12.; [16] M. L. Gimeno et al., “Pluripotent nontumorigenic adipose tissue-derived muse cells have immunomodulatory capacity mediated by transforming growth factor-β1,” STEM CELLS Translational Medicine, vol. 6, no. 1, pp. 161–173, Ago., 2016. doi:10.5966/sctm.2016-0014.; [17] A. A. Simerman, J. D. Phan, D. A. Dumesic y G. D. Chazenbalk, “Muse cells: Nontumorigenic pluripotent stem cells present in adult tissues—A paradigm shift in tissue regeneration and evolution,” Stem Cells International, vol. 2016, pp. 1–8, Dic., 2016. doi:10.1155/2016/1463258.; [18] R. Lovell-Badge, “The future for stem cell research,” Nature, vol. 414, no. 6859, pp. 88–91, Nov., 2001. doi:10.1038/35102150.; [19] M. Dezawa, “Muse cells provide the pluripotency of mesenchymal stem cells: Direct contribution of muse cells to tissue regeneration, “ Cell Transplant, vol. 25, no. 5, pp. 849-861, Feb., 2016. doi:10.3727/096368916X690881.; [20] I. L. Weissman y J. A. Shizuru, “The origins of the identification and isolation of hematopoietic stem cells, and their capability to induce donor-specific transplantation tolerance and treat autoimmune diseases,” Blood, vol. 112, no. 9, pp. 3543–3553, Oct., 2008. doi:10.1182/blood-2008-08-078220.; [21] Y. Kuroda, M. Kitada, S. Wakao y M. Dezawa, “Bone marrow mesenchymal cells: How do they contribute to tissue repair and are they really stem cells?,” Archivum Immunologiae et Therapiae Experimentalis, vol. 59, no. 5, pp. 369– 378, Jul., 2011. doi:10.1007/s00005-011-0139-9.; [22] Cell Culture Basics Handbook, Thermo Fischer Scientific Inc., Waltham, MA, USA, 2016, p. 19.; [23] L. A. Flanagan, B. Ziaeian, T. Palmer y P. H. Schwartz, “Immunocytochemical Analysis of Stem Cells,” en Human Stem Cell Manual. USA: Elsevier, 2007, cap. 9, pp. 108–126. doi:10.1016/B978-012370465-8/50014-4.; [24] S. Marchenko y L. Flanagan, “Immunocytochemistry: Human Neural Stem Cells,” Journal of Visualized Experiments, no. 7, Ago., 2007. doi:10.3791/267.; [25] P. A. Campbell, “Alkaline Phosphatase Staining,” Bio-Protocol, vol. 4, no. 5, Mar., 2014. doi:10.21769/BioProtoc.1060.; [26] T. Tian, R. Zhang, Y. Yang, Q. Liu, D. Li y X. Pan, “Muse cells derived from dermal tissues can differentiate into melanocytes,” Cellular Reprogramming, vol. 19, no. 2, pp. 116-122, Abr., 2017. doi:10.1089/cell.2016.0032.; [27] H. Yabuki, S. Wakao, Y. Kushida, M. Dezawa y Y.Okada, “Human multilineagedifferentiating stress-enduring cells exert pleiotropic effects to ameliorate acute lung ischemia-reperfusion injury in a rat model,” Cell Transplant, vol. 27, no. 6, pp. 979-993, Abr., 2018. doi:10.1177/0963689718761657.; [28] N. Uchida et al., “Beneficial effects of systemically administered human muse cells in adriamycin nephropathy,” Journal of the American Society of Nephrology, vol. 28, no. 10, pp. 2946-2960, Jul., 2017. doi:10.1681/asn.2016070775.; [29] J. Cao, Z. Yang, R. Xiao, y B. Pan, “Regenerative potential of pluripotent nontumorgenetic stem cells: Multilineage differentiating stress enduring cells (Muse cells),” Regenerative Therapy, vol. 15, pp. 92–96, Dic., 2020. doi:10.1016/j.reth.2020.04.011.; [30] F. Motoe, T. Yamauchi, K. Yamasaki y S. Aiba, “LB1606 Retainability of pluripotency and viability of multilineage-differentiating stress enduring (Muse) cells after repeated cryopreservation,” Journal of Investigative Dermatology, vol. 138, no. 9, p. B23, Sep., 2018. doi:10.1016/j.jid.2018.06.147.; [31] T. Yamauchi, K. Yamasaki, K. Tsutiyama, S. Koike y S. Aiba, “904 Adipose multilineage-differentiating stress enduring (Muse) cell maintain pluripotency regardless of donors’ age,” Journal of Investigative Dermatology, vol. 137, no. 5, p. S156, Mayo, 2017. doi:10.1016/j.jid.2017.02.931.; [32] P. A. Neuta Arciniegas, “Implementación de un modelo pre-clínico con aloinjertos de células mesenquimales diferenciadas para prevenir la insuficiencia cardiaca crónica secundaria a un infarto agudo de miocardio,” Tesis Ph.D., Fac. Salud, Dpto. Ciencias Fisiológicas, Prog. Doctorado en Ciencias Biomédicas, Univ. del Valle, Santiago de Cali, Valle, 2013.; Universidad Autónoma de Occidente (UAO); Repositorio Educativo Digital; (2021) S. 8639

Hochschulschrift

Wie komme ich dran?

Las células diferenciables a multilinaje resistentes al estrés (Muse) son una población celular descubierta en el 2010, que está presente en los distintos tejidos mesenquimales. Por su pluripotencialidad, capacidad de integración tisular y baja tumorigénesis, las células Muse prometen ser una forma de tratamiento eficiente en el campo de la medicina regenerativa para diversas enfermedades. Sin embargo, la mayoría de protocolos establecidos para su aislamiento involucran el uso del marcador celular SSEA-3 con la técnica de clasificación de células activada por fluorescencia (FACS), que requiere el uso de un citómetro de flujo que no es un equipo de rutina. El propósito de esta investigación fue verificar la existencia de células tipo Muse en rata y estandarizar un protocolo para su obtención sin utilizar la técnica de FACS. Se partió de un cultivo de células madre mesenquimales (MSC) que fueron sometidas a 4, 8 y 12 horas de incubación con tripsina a largo plazo (ITLP). El tiempo de 8 horas de ITLP demostró ser el más apropiado debido a que se lograba aislar las células tipo Muse de las MSC, sin perjudicar su crecimiento. La obtención de células tipo Muse se confirmó mediante una caracterización por inmunocitoquímica, donde los cúmulos y células resistentes al estrés demostraron la presencia del marcador SSEA-3. El protocolo establecido en este documento representa un método más asequible y reproducible que sirve como punto de partida para llevar a cabo más estudios con esta población celular. ; Multi-lineage stress-enduring (Muse) cells are a cell population that was discovered in 2010 that is present in the different mesenchymal tissues. Because of their pluripotency, tissue integration capacity and low tumorigenesis, Muse cells promise to be a form of efficient treatment in the field of regenerative medicine for various diseases. However, the majority of established protocols for their isolation involve the use of the SSEA-3 cell marker along with the fluorescence-activated cell sorting (FACS) technique, which ...

Titel:	Estandarización de un protocolo para la obtención de células diferenciables a multilinaje por medio de estrés químico
Autor/in / Beteiligte Person:	Giraldo Palacios, Alvaro José ; Neuta Arciniegas, Paola Andrea ; Universidad Autónoma de Occidente (UAO)
Link:	https://hdl.handle.net/10614/13024 https://doi.org/10.1073/pnas.0911647107 https://doi.org/10.1371/journal.pone.0064752 https://doi.org/10.1089/cell.2012.0080 https://doi.org/10.3727/096368916X693662 https://doi.org/10.5966/sctm.2014-0181 https://doi.org/10.1002/stem.2206 https://doi.org/10.1016/j.jid.2017.06.021 https://doi.org/10.1016/j.jgeb.2018.09.003 https://doi.org/10.1016/j.reth.2018.10.005 https://doi.org/10.1038/nprot.2013.076
Quelle:	[1] Y. Kuroda et al., “Unique multipotent cells in adult human mesenchymal cell populations,” Proceedings of the National Academy of Sciences, vol. 107, no. 19, pp. 8639–8643, Mayo, 2010. doi:10.1073/pnas.0911647107.; [2] S. Heneidi et al., “Awakened by cellular tress: Isolation and characterization of a novel population of pluripotent stem cells derived from human adipose tissue,” PLOS ONE, vol. 8, no. 6, p. e64752, Jun., 2013. doi:10.1371/journal.pone.0064752; [3] Z. Yang et al., “Isolation and characterization of ssea3+ stem cells derived from goat skin fibroblasts,” Cellular Reprogramming, vol. 15, no. 3, pp. 195–205, Jun., 2013. doi:10.1089/cell.2012.0080.; [4] M. Iseki et al., “Human muse cells, nontumorigenic phiripotent-like stem cells, have liver regeneration capacity through specific homing and cell replacement in a mouse model of liver fibrosis,” Cell Transplantation, vol. 26, no. 5, pp. 821– 840, Mayo, 2017. doi:10.3727/096368916X693662.; [5] K. Kinoshita et al., “Therapeutic potential of adipose-derived ssea-3-positive muse cells for treating diabetic skin ulcers,” STEM CELLS Translational Medicine, vol. 4, no. 2, pp. 146–155, Ene., 2015. doi:10.5966/sctm.2014-0181.; [6] H. Uchida et al., “Transplantation of unique subpopulation of fibroblasts, muse cells, ameliorates experimental stroke possibly via robust neuronal differentiation,” STEM CELLS, vol. 34, no. 1, pp. 160–173, Ene., 2015. doi:10.1002/stem.2206.; [7] T. Yamauchi, K. Yamasaki, K. Tsuchiyama, S. Koike y S. Aiba, “The potential of muse cells for regenerative medicine of skin: Procedures to reconstitute skin with muse cell-derived keratinocytes, fibroblasts, and melanocytes,” Journal of Investigative Dermatology, vol. 137, no. 12, pp. 2639–2642, Dic., 2017. doi:10.1016/j.jid.2017.06.021.; [8] A. M. Fouad et al., “In vitro differentiation of human multilineage differentiating stress-enduring (Muse) cells into insulin producing cells,” Journal of Genetic Engineering and Biotechnology, vol. 16, no. 2, pp. 433–440, Dic., 2018. doi:10.1016/j.jgeb.2018.09.003.; [9] E. Toyoda et al., “Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue,” Regenerative Therapy, vol. 10, pp. 17–26, Jun., 2019. doi:10.1016/j.reth.2018.10.005.; [10] M. Dezawa, “Clinical Trials of Muse Cells” en Muse Cells. Japón: Springer Japan, 2018, cap. 17, p. 308.; [11] Y. Kuroda, S. Wakao, M. Kitada, T. Murakami, M. Nojima y M. Dezawa, “Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells,” Nature Protocols, vol. 8, no. 7, pp. 1391–1415, Jun., 2013. doi:10.1038/nprot.2013.076.; [12] K. Tatsumi, Y. Kushida, S. Wakao, Y. Kuroda y M. Dezawa, “Protocols for Isolation and Evaluation of Muse Cells,” en Advances in Experimental Medicine and Biology. Japón: Springer Japan, 2018, pp. 69–101. doi:10.1007/978-4- 431-56847-6_4.; [13] S. C. Fisch et al., “Pluripotent nontumorigenic multilineage differentiating stress enduring cells (Muse cells): A seven-year retrospective,” Stem Cell Research & Therapy, vol. 8, no. 1, Oct., 2017. doi:10.1186/s13287-017-0674-3.; [14] S. A. Przyborski, “Differentiation of human embryonic stem cells after transplantation in immune-deficient mice,” STEM CELLS, vol. 23, no. 9, pp. 1242–1250, Oct., 2005. doi:10.1634/stemcells.2005-0014.; [15] A. A. Simerman, D. A. Dumesic y G. D. Chazenbalk, “Pluripotent muse cells derived from human adipose tissue: A new perspective on regenerative medicine and cell therapy,” Clinical and Translational Medicine, vol. 3, no. 1, pp. 3-12, Mayo, 2014. doi:10.1186/2001-1326-3-12.; [16] M. L. Gimeno et al., “Pluripotent nontumorigenic adipose tissue-derived muse cells have immunomodulatory capacity mediated by transforming growth factor-β1,” STEM CELLS Translational Medicine, vol. 6, no. 1, pp. 161–173, Ago., 2016. doi:10.5966/sctm.2016-0014.; [17] A. A. Simerman, J. D. Phan, D. A. Dumesic y G. D. Chazenbalk, “Muse cells: Nontumorigenic pluripotent stem cells present in adult tissues—A paradigm shift in tissue regeneration and evolution,” Stem Cells International, vol. 2016, pp. 1–8, Dic., 2016. doi:10.1155/2016/1463258.; [18] R. Lovell-Badge, “The future for stem cell research,” Nature, vol. 414, no. 6859, pp. 88–91, Nov., 2001. doi:10.1038/35102150.; [19] M. Dezawa, “Muse cells provide the pluripotency of mesenchymal stem cells: Direct contribution of muse cells to tissue regeneration, “ Cell Transplant, vol. 25, no. 5, pp. 849-861, Feb., 2016. doi:10.3727/096368916X690881.; [20] I. L. Weissman y J. A. Shizuru, “The origins of the identification and isolation of hematopoietic stem cells, and their capability to induce donor-specific transplantation tolerance and treat autoimmune diseases,” Blood, vol. 112, no. 9, pp. 3543–3553, Oct., 2008. doi:10.1182/blood-2008-08-078220.; [21] Y. Kuroda, M. Kitada, S. Wakao y M. Dezawa, “Bone marrow mesenchymal cells: How do they contribute to tissue repair and are they really stem cells?,” Archivum Immunologiae et Therapiae Experimentalis, vol. 59, no. 5, pp. 369– 378, Jul., 2011. doi:10.1007/s00005-011-0139-9.; [22] Cell Culture Basics Handbook, Thermo Fischer Scientific Inc., Waltham, MA, USA, 2016, p. 19.; [23] L. A. Flanagan, B. Ziaeian, T. Palmer y P. H. Schwartz, “Immunocytochemical Analysis of Stem Cells,” en Human Stem Cell Manual. USA: Elsevier, 2007, cap. 9, pp. 108–126. doi:10.1016/B978-012370465-8/50014-4.; [24] S. Marchenko y L. Flanagan, “Immunocytochemistry: Human Neural Stem Cells,” Journal of Visualized Experiments, no. 7, Ago., 2007. doi:10.3791/267.; [25] P. A. Campbell, “Alkaline Phosphatase Staining,” Bio-Protocol, vol. 4, no. 5, Mar., 2014. doi:10.21769/BioProtoc.1060.; [26] T. Tian, R. Zhang, Y. Yang, Q. Liu, D. Li y X. Pan, “Muse cells derived from dermal tissues can differentiate into melanocytes,” Cellular Reprogramming, vol. 19, no. 2, pp. 116-122, Abr., 2017. doi:10.1089/cell.2016.0032.; [27] H. Yabuki, S. Wakao, Y. Kushida, M. Dezawa y Y.Okada, “Human multilineagedifferentiating stress-enduring cells exert pleiotropic effects to ameliorate acute lung ischemia-reperfusion injury in a rat model,” Cell Transplant, vol. 27, no. 6, pp. 979-993, Abr., 2018. doi:10.1177/0963689718761657.; [28] N. Uchida et al., “Beneficial effects of systemically administered human muse cells in adriamycin nephropathy,” Journal of the American Society of Nephrology, vol. 28, no. 10, pp. 2946-2960, Jul., 2017. doi:10.1681/asn.2016070775.; [29] J. Cao, Z. Yang, R. Xiao, y B. Pan, “Regenerative potential of pluripotent nontumorgenetic stem cells: Multilineage differentiating stress enduring cells (Muse cells),” Regenerative Therapy, vol. 15, pp. 92–96, Dic., 2020. doi:10.1016/j.reth.2020.04.011.; [30] F. Motoe, T. Yamauchi, K. Yamasaki y S. Aiba, “LB1606 Retainability of pluripotency and viability of multilineage-differentiating stress enduring (Muse) cells after repeated cryopreservation,” Journal of Investigative Dermatology, vol. 138, no. 9, p. B23, Sep., 2018. doi:10.1016/j.jid.2018.06.147.; [31] T. Yamauchi, K. Yamasaki, K. Tsutiyama, S. Koike y S. Aiba, “904 Adipose multilineage-differentiating stress enduring (Muse) cell maintain pluripotency regardless of donors’ age,” Journal of Investigative Dermatology, vol. 137, no. 5, p. S156, Mayo, 2017. doi:10.1016/j.jid.2017.02.931.; [32] P. A. Neuta Arciniegas, “Implementación de un modelo pre-clínico con aloinjertos de células mesenquimales diferenciadas para prevenir la insuficiencia cardiaca crónica secundaria a un infarto agudo de miocardio,” Tesis Ph.D., Fac. Salud, Dpto. Ciencias Fisiológicas, Prog. Doctorado en Ciencias Biomédicas, Univ. del Valle, Santiago de Cali, Valle, 2013.; Universidad Autónoma de Occidente (UAO); Repositorio Educativo Digital; (2021) S. 8639
Veröffentlichung:	Universidad Autónoma de Occidente (UAO) ; Ingeniería Biomédica ; Departamento de Automática y Electrónica ; Facultad de Ingeniería ; Cali, 2021
Medientyp:	Hochschulschrift
DOI:	10.1073/pnas.0911647107
Schlagwort:	Ingeniería Biomédica Células Muse Incubación con tripsina a largo plazo Inmunocitoquímica Células madre Medicina regenerativa Ingeniería de tejidos Stem cells Regenerative medicine Tissue engineering Time: Universidad Autónoma de Occidente. Calle 24 115-85. Km 2 vía Cali - Jamundí
Sonstiges:	Nachgewiesen in: BASE Sprachen: Spanish; Castilian Collection: Repositorio Educativo Digital Universidad Autónoma de Occidente (RED UAO) Document Type: bachelor thesis File Description: 48 páginas; application/pdf Language: Spanish; Castilian Rights: Derechos reservados - Universidad Autónoma de Occidente, 2021 ; https://creativecommons.org/licenses/by-nc-nd/4.0/ ; info:eu-repo/semantics/openAccess ; Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)

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