Cloning and expression of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity
In: Journal of Bacteriology, Jg. 172 (1990-10-01), Heft 10, S. 5783-5788
Online
serialPeriodical
Zugriff:
A gene of Staphylococcus aureus PS47 encoding lytic activity was cloned and expressed in Escherichia coli. Deletion analysis of a recombinant plasmid carrying a 7.4-kilobase-pair fragment (kbp) of S. aureus DNA suggested that the gene was located within a 2.5-kbp EcoRI-XbaI fragment. Analysis of extracts of E. coli harboring recombinant plasmids on denaturing polyacrylamide gels containing purified cell walls of S. aureus showed a clearing zone by a polypeptide of apparent Mr 23,000. The release of dinitrophenylalanine but not reducing groups from purified cell walls by a cell extract of recombinant E. coli suggested that we had cloned an N-acetylmuramyl-L-alanine amidase.
Titel: |
Cloning and expression of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity
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Autor/in / Beteiligte Person: | Jayaswal, R K ; Lee, Y I ; Wilkinson, B J |
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Zeitschrift: | Journal of Bacteriology, Jg. 172 (1990-10-01), Heft 10, S. 5783-5788 |
Veröffentlichung: | 1990 |
Medientyp: | serialPeriodical |
ISSN: | 0021-9193 (print) ; 1098-5530 (print) |
DOI: | 10.1128/jb.172.10.5783-5788.1990 |
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