Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis
In: Mechanisms of Development, Jg. 73 (1998-05-01), Heft 2, S. 159-168
Online
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Zugriff:
cDNAs specific to vegetal poles of Xenopusgastrula embryos were used as a probe to screen a gastrula vegetal pole cDNA library. One of the novel clones isolated had an RNA expression pattern consistent with it being a component of germ plasm and it was thus named Xpat(Xenopusprimordial germ cell associated transcript). The open reading frame encodes a 35 kDa protein with no clear homologies. The RNA is localised to the vegetal pole throughout oogenesis and early cleavage. During gastrulation cells containing this message move internally and at tailbud stages they migrate in an antero-dorsal direction. XpatmRNA is not detectable once the dorsal mesentery forms. We show that the 3′-UTR is required and is sufficient for localisation of exogenous RNA to the vegetal pole of oocytes. We propose that XpatUTR-containing transcripts can be localised by the Vg1or late pathway of mRNA localisation during stage III of oogenesis, but endogenous Xpatappears to be localised earlier by a mitochondrial cloud mechanism similar to that proposed for Xcat-2.
Titel: |
Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis
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Autor/in / Beteiligte Person: | Hudson, Clare ; Woodland, Hugh R |
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Zeitschrift: | Mechanisms of Development, Jg. 73 (1998-05-01), Heft 2, S. 159-168 |
Veröffentlichung: | 1998 |
Medientyp: | serialPeriodical |
ISSN: | 0925-4773 (print) ; 1872-6356 (print) |
DOI: | 10.1016/S0925-4773(98)00047-1 |
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