Differential Activation of Phospholipase D by VPAC and PAC1Receptors
In: Annals of the New York Academy of Sciences, Jg. 921 (2000-12-01), Heft 1, S. 175-185
Online
serialPeriodical
Zugriff:
To investigate the phospholipase D (PLD) responses of the VIPPACAP receptors, VPAC1 and VPAC2, and the PACAP-specific PAC1 receptors (short and "hop" intracellular loop 3 (i3) splice variants), stable CHO cell lines expressing similar levels of each wildtype receptor were generated (except for the VPAC1receptor clone which showed considerably lower expression and lesser responses in signalling assays). All clones caused activation of PLD in response to agonists, as monitored by [3H]phosphatidylbutanol production. The PLD responses of the PAC1"hop", but not the "null" receptor, were sensitive to the ARF inhibitor, brefeldin A (BFA) (as were VPAC1and VPAC2responses). Chimeric constructs of VPAC2receptors containing i3 of either PAC1hop or PAC1null receptors were transiently expressed in COS 7 cells and PLD responses were measured. Only the PLD response of the hop construct was sensitive to BFA. This suggests that i3 motifs in certain Group II GPCRs may play a key role in determining their linkage to ARF-dependent PLD activation.
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Differential Activation of Phospholipase D by VPAC and PAC1Receptors
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Autor/in / Beteiligte Person: | McCULLOCH, D. A. ; LUTZ, E. M. ; JOHNSON, M. S. ; MacKENZIE, C. J. ; MITCHELL, R. |
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Zeitschrift: | Annals of the New York Academy of Sciences, Jg. 921 (2000-12-01), Heft 1, S. 175-185 |
Veröffentlichung: | 2000 |
Medientyp: | serialPeriodical |
ISSN: | 0077-8923 (print) ; 1749-6632 (print) |
DOI: | 10.1111/j.1749-6632.2000.tb06964.x |
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