Contribution de la PCR à la surveillance microbiologique et épidémiologique des méningites bactériennes aiguës en Afrique : à propos de l'expérience d'un transfert de technologie réussi au centre Muraz de ... (French)
In: Revue Francaise des Laboratoires, Jg. 2005 (2005-04-01), Heft 372, S. 33-40
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Summary: The recognition of Neisseria meningitidis (Nm) W135 and Streptococcus pneumoniae (Sp) as major causes of acute bacterial meningitis (ABM) emphasizes the need for field-based methods to rapidly and accurately determine the aetiology. The polymerase chain reaction (PCR) technology developed at the Pasteur Institute-Paris was transferred to a reference laboratory in Bobo Dioulasso, Burkina Faso. Cerebrospinal fluid (CSF) samples were collected from suspected acute bacterial meningitis (ABM) patients from five districts in Burkina Faso and Togo. Between april 2002 and june 2003, 1 645 CSF specimens were tested by PCR and 560 pathogens were identified including 281 Nm (50 %), 193 Sp (34 %), and 86 Haemophilus influenzae (Hi) (15 %). Compared to culture, PCR sensitivity was 95 %, 75 %, and 81 % for Nm, Sp, and Hi, respectively while specificity was ≥ 95 % for all pathogens. Of the 560 PCR-positive samples, 383 were also tested by culture and 263 (69 %) identified a pathogen; of the 288 culture-positive samples, 283 were tested by PCR and 263 (93 %) yielded a pathogen. The specificity of crgA, siaD and mynB PCR assays to identify Nm isolates and determine Nm serogroup was 100 % ; The kappa coefficient between samples tested in Burkina Faso and Paris was 0.80. PCR for ABM can be successfully implemented in a Sub-Saharan African countries field setting and allowed identification of substantially more cases than culture alone. [Copyright &y& Elsevier]
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Titel: |
Contribution de la PCR à la surveillance microbiologique et épidémiologique des méningites bactériennes aiguës en Afrique : à propos de l'expérience d'un transfert de technologie réussi au centre Muraz de ... (French)
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Autor/in / Beteiligte Person: | Njanpop-Lafourcade, Berthe-Marie ; Traoré, Yves ; Aguilera, Jean-François ; Sanou, Oumarou ; Lourd, Mathilde ; Giorgini, Dario ; Silva, Alfred Da ; Gessner, Bradford D. ; Taha, Muhamed-Kheir |
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Zeitschrift: | Revue Francaise des Laboratoires, Jg. 2005 (2005-04-01), Heft 372, S. 33-40 |
Veröffentlichung: | 2005 |
Medientyp: | academicJournal |
ISSN: | 0338-9898 (print) |
DOI: | 10.1016/S0338-9898(05)80196-9 |
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