Establishment and characterization of a human uterine endometrial undifferentiated carcinoma cell line, TMG-L.
In: Human Cell, Jg. 16 (2003-03-01), Heft 1, S. 31-38
Online
academicJournal
Zugriff:
A new cell line of human uterine endometrial undifferentiated carcinoma, designated as TMG-L, was established from the metastatic lymph node of 56-year-old patient. TMG-L cells have been cultured with Ham's F-12 medium supplemented with 10% FCS and grew as a loosely adherent monolayer with polygonal or spindle-shaped cells exhibiting poor cellcell contact and piled up against each other, showing a tendency to grow as floating cells. The doubling time of this cell line was about 48 hours, and chromosomal analysis revealed aneuploidy at passage 25. The cells formed tumors in SCID mouse, the histology of which was similar to that of undifferentiated carcinoma component of primary tumor. TMG-L cells showed the loss of expression and membranous localization of either E-cadherin or α-catenin, implied corresponding loss of their adhesive function. And this dysfunction implicated the biological aggressive behavior of uterine endometrial undifferentiated carcinoma. This cell line appears to provide a useful system for studying uterine undifferentiated Carcinoma in vivo and in vitro. [ABSTRACT FROM AUTHOR]
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Titel: |
Establishment and characterization of a human uterine endometrial undifferentiated carcinoma cell line, TMG-L.
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Autor/in / Beteiligte Person: | Hasegawa, Kiyoshi ; Suzuki, Machiko ; Ishikawa, Kunimi ; Yasue, Akira ; Kato, Rina ; Nakamura, Azumi ; Kuroki, Jun ; Udagawa, Yasuhiro |
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Zeitschrift: | Human Cell, Jg. 16 (2003-03-01), Heft 1, S. 31-38 |
Veröffentlichung: | 2003 |
Medientyp: | academicJournal |
ISSN: | 0914-7470 (print) |
DOI: | 10.1111/j.1749-0774.2003.tb00126.x |
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