Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina).
In: BMC Microbiology, Jg. 12 (2012-01-02), Heft Suppl 1, S. 1-13
Online
academicJournal
Zugriff:
Background: Wolbachia is a genus of endosymbiotic a-Proteobacteria infecting a wide range of arthropods and filarial nematodes. Wolbachia is able to induce reproductive abnormalities such as cytoplasmic incompatibility (CI), thelytokous parthenogenesis, feminization and male killing, thus affecting biology, ecology and evolution of its hosts. The bacterial group has prompted research regarding its potential for the control of agricultural and medical disease vectors, including Glossina spp., which transmits African trypanosomes, the causative agents of sleeping sickness in humans and nagana in animals. Results: In the present study, we employed a Wolbachia specific 16S rRNA PCR assay to investigate the presence of Wolbachia in six different laboratory stocks as well as in natural populations of nine different Glossina species originating from 10 African countries. Wolbachia was prevalent in Glossina morsitans morsitans, G. morsitans centralis and G. austeni populations. It was also detected in G. brevipalpis, and, for the first time, in G. pallidipes and G. palpalis gambiensis. On the other hand, Wolbachia was not found in G. p. palpalis, G. fuscipes fuscipes and G. tachinoides. Wolbachia infections of different laboratory and natural populations of Glossina species were characterized using 16S rRNA, the wsp (Wolbachia Surface Protein) gene and MLST (Multi Locus Sequence Typing) gene markers. This analysis led to the detection of horizontal gene transfer events, in which Wobachia genes were inserted into the tsetse flies fly nuclear genome. Conclusions: Wolbachia infections were detected in both laboratory and natural populations of several different Glossina species. The characterization of these Wolbachia strains promises to lead to a deeper insight in tsetse flies- Wolbachia interactions, which is essential for the development and use of Wolbachia-based biological control methods. [ABSTRACT FROM AUTHOR]
Copyright of BMC Microbiology is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina).
|
---|---|
Autor/in / Beteiligte Person: | Doudoumis, Vangelis ; Tsiamis, George ; Wamwiri, Florence ; Brelsfoard, Corey ; Alam, Uzma ; Aksoy, Emre ; Dalaperas, Stelios ; Abd-Alla, Adly ; Ouma, Johnson ; Takac, Peter ; Aksoy, Serap ; Bourtzis, Kostas |
Link: | |
Zeitschrift: | BMC Microbiology, Jg. 12 (2012-01-02), Heft Suppl 1, S. 1-13 |
Veröffentlichung: | 2012 |
Medientyp: | academicJournal |
ISSN: | 1471-2180 (print) |
DOI: | 10.1186/1471-2180-12-S1-S3 |
Schlagwort: |
|
Sonstiges: |
|