PW1 gene/paternally expressed gene 3 (PW1/Peg3) identifies multiple adult stem and progenitor cell populations.
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 108 (2011-07-12), Heft 28, S. 11470-11475
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Zugriff:
A variety of markers are invaluable for identifying and purifying stem/progenitor cells. Here we report the generation of a murine reporter line driven by Pw1 that reveals cycling and quiescent progenitor/stem cells in all adult tissues thus far examined, including the intestine, blood, testis, central nervous system, bone, skeletal muscle, and skin. Neurospheres generated from the adult PW1-reporter mouse show near 100% reporter-gene expression following a single passage. Furthermore, epidermal stem cells can be purified solely on the basis of reporter-gene expression. These cells are clonogenic, repopulate the epidermal stem-cell niches, and give rise to new hair follicles. Finally, we demonstrate that only PW1 reporter-expressing epidermal cells give rise to follicles that are capable of self-renewal following injury. Our data demonstrate that PW1 serves as an invaluable marker for competent self-renewing stem cells in a wide array of adult tissues, and the PW1-reporter mouse serves as a tool for rapid stem cell isolation and characterization. [ABSTRACT FROM AUTHOR]
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PW1 gene/paternally expressed gene 3 (PW1/Peg3) identifies multiple adult stem and progenitor cell populations.
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Autor/in / Beteiligte Person: | Besson, Vanessa ; Smeriglio, Piera ; Wegener, Amélie ; Relaix, Frédéric ; Oumesmar, Brahim Nait ; Sassoon, David A. ; Marazzi, Giovanna |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 108 (2011-07-12), Heft 28, S. 11470-11475 |
Veröffentlichung: | 2011 |
Medientyp: | academicJournal |
ISSN: | 0027-8424 (print) |
DOI: | 10.1073/pnas.1103873108 |
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