Characterisation of Curtobacterium flaccumfaciens Pathovars by AFLP, rep-PCR and Pulsed-Field Gel Electrophoresis.
In: European Journal of Plant Pathology, Jg. 109 (2003-10-01), Heft 8, S. 817-825
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Zugriff:
The bacterial species Curtobacterium flaccumfaciens encompasses a group of closely related phytopathogens subdivided into pathovars that exhibit differences in host range. To date, reliable differentiation of pathovars and identification of unknown isolates to the pathovar-level can only be achieved on the basis of host testing. In this study, representative strains from C. flaccumfaciens pathovars and related species were examined using a range of genomic fingerprinting techniques to ascertain their application as additional or potential alternatives to host testing. Amplified fragment length polymorphism (AFLP) and repetitive sequence polymerase chain reaction (rep-PCR) analyses enabled the categorisation of some, but not all strains, in their respective pathovars. In contrast, all strains were correctly assigned to pathovars using HindIII and XbaI macro-restriction digests in conjunction with pulsed-field gel electrophoresis (PFGE). Fingerprints generated by PFGE not only supported the current pathovar rankings within C. flaccumfaciens, they also identified subgroups within pathovars flaccumfaciens, oortii and poinsettiae. [ABSTRACT FROM AUTHOR]
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Titel: |
Characterisation of Curtobacterium flaccumfaciens Pathovars by AFLP, rep-PCR and Pulsed-Field Gel Electrophoresis.
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Autor/in / Beteiligte Person: | Patricia M. Guimar aes ; Smith, Julian J. ; Palmano, Sabrina ; Saddler, Gerry S. |
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Zeitschrift: | European Journal of Plant Pathology, Jg. 109 (2003-10-01), Heft 8, S. 817-825 |
Veröffentlichung: | 2003 |
Medientyp: | academicJournal |
ISSN: | 0929-1873 (print) |
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