Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli.
In: Microbial Cell Factories, Jg. 23 (2024-02-16), Heft 1, S. 1-11
Online
academicJournal
Zugriff:
Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-10 was constructed to transform corn cob hydrolysate into 1,2,4-butanetriol, a platform substance with diversified applications. To eliminate catabolite repression and enhance NADPH supply for alcohol dehydrogenase YqhD catalyzed 1,2,4-butanetriol generation, ptsG encoding glucose transporter EIICB Glc and pgi encoding phosphoglucose isomerase were deleted. With four heterologous enzymes including xylose dehydrogenase, xylonolactonase, xylonate dehydratase, α-ketoacid decarboxylase and endogenous YqhD, E. coli BT-10 can produce 36.63 g/L 1,2,4-butanetriol with a productivity of 1.14 g/[L·h] using xylose as substrate. When corn cob hydrolysate was used as the substrate, 43.4 g/L 1,2,4-butanetriol was generated with a productivity of 1.09 g/[L·h] and a yield of 0.9 mol/mol. With its desirable characteristics, E. coli BT-10 is a promising strain for commercial 1,2,4-butanetriol production. [ABSTRACT FROM AUTHOR]
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Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli.
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Autor/in / Beteiligte Person: | Li, Ping ; Wang, Mengjiao ; Di, Haiyan ; Du, Qihang ; Zhang, Yipeng ; Tan, Xiaoxu ; Xu, Ping ; Gao, Chao ; Jiang, Tianyi ; Lü, Chuanjuan ; Ma, Cuiqing |
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Zeitschrift: | Microbial Cell Factories, Jg. 23 (2024-02-16), Heft 1, S. 1-11 |
Veröffentlichung: | 2024 |
Medientyp: | academicJournal |
ISSN: | 1475-2859 (print) |
DOI: | 10.1186/s12934-024-02317-0 |
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